山西农业大学学报（自然科学版）2016，Vol.36Issue(7)：477-482,6.
基于荧光检测的糜子 Genic-SSR PCR 体系优化
The optimization of PCR reaction system for Genic-SSR analysis in broomcorn millet
摘要
Abstract
Objective]The fluorescent PCR reaction system for Genic-SSR analysis was optimized by using the Frag-ment Analyzer Automatic Capillary Electrophoresis detection system in broomcorn millet.[Methods]Optimization was based on L1 6 (4 4 )orthogonal design,with different concentrations of Taq DNA Polymerase,Mg2+ ,dNTPs and prim-ers.[Results]The order of the effects for the system was:Mg2+ >primer>dNTPs>Taq DNA Polymerase.Through the orthogonal experimental design,a 20 μL reaction system was established,which contained a 0.2 mol·L-1 dNTPs, 0.2 μmmol·L-1 SSR primer,1.5 mmol·L-1 Mg2+ ,and 0.5 U Taq DNA Polymerase.[Conclusion]This optimal PCR reaction system could be used for genetic clustering analysis of germplasm with Genic-SSR markers and for further de-velopment of molecular markers for marker-assisted breeding in broomcorn millet.关键词
糜子/Genic-SSR/体系优化/正交试验设计Key words
Broomcorn millet/Genic-SSR/PCR reaction system/Orthogonal design分类
农业科技引用本文复制引用
李耀深,曹鑫,段明,王俊杰,张彬,李红英,侯思宇,韩渊怀..基于荧光检测的糜子 Genic-SSR PCR 体系优化[J].山西农业大学学报（自然科学版）,2016,36(7):477-482,6.基金项目
山西农业大学科技创新育种基金(2013-2016) （2013-2016）
山西特色植物基因资源开发与利用平台项目(2012091004-0103) （2012091004-0103）
