山西农业大学学报（自然科学版）2016，Vol.36Issue(7)：489-494,6.
亳菊试管嫁接苗褐变生理机制研究
Study on physiological mechanism of browning in vitro grafting of Dendranthema morifolium (Ra-mat.)Tzvel ‘Boju’cv.Nov.
摘要
Abstract
Objective]To reveal the mechanism of browning in the process of micro-graft in vitro in Dendranthema morifolium (Ramat.)Tzvel ‘Boju’cv.Nov.[Methods]The Dendranthema morifolium (Ramat.)Tzvel ‘Boju’cv. Nov.was used as explant,and the total phenolic content,polyphenol oxidase activity and browning index of in vitro browned grafts were detected by Folin Method and Polyphenol oxidase Method.[Results]The results showed that with the increasing of browning series,the browning situation was more serious.The browning ratio of series 4(89.7%) was significantly greater than other browning series.The greater the browning series was,the total phenolic content was decreasing,meanwhile the polyphenol oxidase activity in the in vitro grafts was increased.There was a linear neg-ative correlation between the browning series and the explant total phenolic content,and there was a linear positive cor-relation between the browning series and the polyphenol oxidase activity.[Conclusion]This study providing the theory basis for increasing the efficiency of Dendranthema morifolium (Ramat.)Tzvel ‘Boju’cv.Nov.in vitro grafts.关键词
菊花/菊试管微嫁接/褐变Key words
Chrysanthemum/Micro-graft in vitro/Browning分类
农业科技引用本文复制引用
纪薇,王瑞,狄娟,刘榕晨,罗尧幸,茹慧玲,杨忠义..亳菊试管嫁接苗褐变生理机制研究[J].山西农业大学学报（自然科学版）,2016,36(7):489-494,6.基金项目
山西省科技基础条件平台建设项目(201509016) （201509016）
国家自然科学基金(31401834) （31401834）
山西农业大学青年拔尖创新人才支持计划(TYIT201401) （TYIT201401）
