山西农业大学学报（自然科学版）2016，Vol.36Issue(7)：510-513,518,5.
微生物混合对纤维素酶活的影响
The influence of mixed microbe to cellulase activity
摘要
Abstract
Objective]In this paper,we mixed Aspergillus niger and Trichoderma reesei together,aimed at providing reference for the coexist of microbial species and the optimization of cellulase proportion .[Methods]Based on the total amount of inoculation 8 mL,we put Trichoderma reesei ,Aspergillus niger ,and mixed strains in 1∶1 into enzyme pro-duction medium respectively,then determined three-component cellulase activity and filter paper activity in the following ten days to analysis growth relationship of microbe and the proportion of components cellulase activity.[Results]It suggested that mixed strains’endoglucanase,exogluconase andβ-glucosidase activity meet the peak in 7 d,4 d and 5 d respectively.After that,exogluconase and β-glucosidase activity rose again.Nevertheless,the three components were lower than single strain in some degree,while the total activity of filter paper enzyme activity was higher than single strain.[Conclusion]In summary,the different proportions of the three components cellulase activity determine the com-plete cellulase system or not,which determines the degree of cellulose degradation.关键词
里氏木霉/黑曲霉/纤维素酶活Key words
Trichoderma reesei/Aspergillus nige/Cellulose enzyme activity分类
生物学引用本文复制引用
郝建宇,侯红萍,郑渊洁..微生物混合对纤维素酶活的影响[J].山西农业大学学报（自然科学版）,2016,36(7):510-513,518,5.基金项目
山西省科技攻关项目(20140311019-2) （20140311019-2）
