山西农业大学学报（自然科学版）2016，Vol.36Issue(9)：609-613,643,6.
大豆高代新品系草甘膦抗性的检测与筛选
Detection and screen of soybean new strains resistant to glyphosate in high generation
摘要
Abstract
Objective]The purpose of this study is to detect the resistance to glyphosate and screen the new glyphosate-resistant soybean materials from Jinda73×RR1 hybrid descendants according to conventional breeding method.[Meth-ods]First,we obtained the homozygous recombinant inbred line F7 group from Jinda73 and RR1 by pedigree method. Second,primers were designed to amplify the CaMV35S promoter,the NOS terminator,the foreign gene cp4-epsps, and the soybean lectin which used as intemal standards.Then,PCR result was confirmed with the test for resistance to glyphosate in the field and quality determination.[Results]The results indicated that CaMV35S promoter,the NOS terminator,and the cp4-epsps were detected in 27 lines,which resistant to glyphosate,There was no cp4-epsps in 5 lines,while they had no resistant to glyphosate.PCR results were not consistent with the test for resistance to glypho-sate in the field in 2 lines.[Conclusion]Spraying glyphosate on 34 strains in the field was consistent 94.1% with PCR detection,there were 4 strains with excellent quality characters in 27 strains which resistant to glyphosate.关键词
杂交/常规育种/抗草甘膦大豆Key words
Hybrid/Conventional breeding/Soybean resistant to glyphosate分类
农业科技引用本文复制引用
王鹏,郭玮,马艳芸,李贵全..大豆高代新品系草甘膦抗性的检测与筛选[J].山西农业大学学报（自然科学版）,2016,36(9):609-613,643,6.基金项目
山西农业大学科技创新项目(20142-03) （20142-03）
山西省财政支持农业科技成果转化资金项目 （）
国家农业综合开发存量资金土地治理项目 （）
