山西农业大学学报（自然科学版）2016，Vol.36Issue(9)：628-632,5.
植物激素对大花蕙兰丛生芽增殖与生长的影响
Effect of phytohormone on the proliferation and growth of clustered shoots in Cymbidium hybridium
摘要
Abstract
Objective]The effects of variety and concentration of several hormones were investigated on proliferation and growt of multiple shoot clumps of Cymbidium hybridium.[Methods]The test-tube plantlet of Cymbidium were used as explants to be cultured in the MS medium with different variety and concentrations of hormones.[Results]The re-sults showed that added different concentrations 6-BA、KT、ZT and NAA(0.2 mg·L-1 )in MS medium could induce differentiation of Multiple shoot clumps,but there were some differences on proliferation rate and growth condition of multiple shoot clumps.The most optimal medium for proliferation and growth of multiple shoot clumps was MS+6-BA3 mg·L-1 + NAA 0.2 mg·L-1 ,with the highest proliferation ratio 3.57.The test-tube plantlet were not appear brown in medium added ZT.The optimal concentration of IBA was 1.0 mg·L-1 when added the same concentration of BA (3 mg·L-1 )and different concentration IBA in MS medium,with the lower proliferation rate compared with added 6-BA and NAA,but the multiple shoot clumps growed vigorously and seedlings directly generated.[Conclusion] There were different influence for different plant hormones on proliferation and growth of multiple shoot clumps and the the optimum medium for it was MS+6-BA 3 mg·L-1 +NAA 0.2 mg·L-1 .关键词
大花蕙兰/丛生芽/植物激素/增殖倍率Key words
Cymbidium hybridium/Clustered shoots/Phytohormone/Proliferation ratio分类
农业科技引用本文复制引用
王育选,任建宏,王鹏丽,赵娟..植物激素对大花蕙兰丛生芽增殖与生长的影响[J].山西农业大学学报（自然科学版）,2016,36(9):628-632,5.基金项目
山西省科技攻关项目(20130311014-1) （20130311014-1）
山西农业大学博士科研启动项目(2012YJ13) （2012YJ13）
