山西农业大学学报(自然科学版)2016,Vol.36Issue(9):639-643,5.
花魔芋实生种子组织培养研究初探
Study on tissue culture of Amorphophallus konjac seed
摘要
Abstract
Objective]This study aimed to explore the best ratio hormones of Amorphophallus konjac seedling seed tis-sue culture,to increase differentiation rate and quality of konjac seed.[Methods]Amorphophallus seeds were taken as explants,with various concentrations BA and NAA were studied.[Results]The results showed that the best callus me-dium of seed was MS+1.0 mg·L-1 6-BA+0.5 mg·L-1 NAA,the ratio of induction was 90%,the weight increase was 7.8;the best redifferentiation bud medium was MS+2.0 mg·L-1 6-BA+0.5 mg·L-1 NAA,the ratio of rediffer-entiation was 100%,the multiplication factor was 4.6. The best rooting medium was 1/2 MS+1 mg·L-1 NAA,the rooting rate was 84%,the number of root was 38. [Conclusion]Finally,high quality seedlings were produced in 4 months,1 5g of Konjac were obtained by hydroponic Technology.关键词
花魔芋/实生种子/配方优化/诱导率/水培Key words
Konjak/Seed/Formulation optimization/Ratio of induction/Hydroponics分类
农业科技引用本文复制引用
段龙飞,郭邦利,陈国爱,刘婷..花魔芋实生种子组织培养研究初探[J].山西农业大学学报(自然科学版),2016,36(9):639-643,5.基金项目
陕西省科学技术研究发展计划项目(2013K01-24) (2013K01-24)