实用医学杂志2016，Vol.32Issue(11)：1760-1763,4.DOI:10.3969/j.issn.1006-5725.2016.11.010
黄芪预处理提高小鼠对肠缺血时间的耐受能力
Tolerance for intestinal ischemia of mouse by astragalus preconditioning
摘要
Abstract
Objective To investigate the protective effects of astragalus preconditioning on the tolerance of ischemia time of mouse small intestine . Methods C57BL/6 mice were randomly divided into 5 groups (n = 7): sham operation group (Sham group),intestinal ischemia reperfusion group (IR group) and astragalus preconditioning group (ASIR group). IR group and ASIR group include 2 sub-groups respectively, specifically, 2 h reperfusion was performed 45 min (ASIR1) and 60 min (ASIR1) after blocking superior mesenteric artery. Intestinal terminal morphology was observed by light microscope after HE coloration . Serum levels of LPS , DAO and intestinal mucosa TNF-α were measured by ELISA. Intestinal Cyto C expression were detected by immunofluorescence. Results Astragalus preconditioning reduces Chiu′s score significantly. Expression of Cyto C was significantly down-regulated in astragalus preconditioning groups, and levels of LPS, DAO and TNF-αsignificantly decreased. The damages in IR2 group is obviously severe than in IR1, but there were no significant differences between this two groups after pretreatment with astragalus. Conclusion Astragalus preconditioning has obvious protective effects to intestinal ischemia reperfusion, and enhances the tolerance to longer time of ischemia.关键词
黄芪/肠缺血再灌注/肠黏膜屏障/二胺氧化酶Key words
Astragalus/Intestinal ischemia reperfusion/Intestinal mucosal barrier/Diamine oxidase引用本文复制引用
罗刚健,陈曦,姚伟锋,陈潮金,吴珊..黄芪预处理提高小鼠对肠缺血时间的耐受能力[J].实用医学杂志,2016,32(11):1760-1763,4.基金项目
广东省科技计划项目 （）
