实用医学杂志2016，Vol.32Issue(12)：1916-1919,4.DOI:10.3969/j.issn.1006-5725.2016.12.007
体内诱导小鼠诱导性多能干细胞向胸腺上皮细胞分化的方法
Generation of thymic epithelial cells in mouse by blastocyst injection of induced pluripotent stem cells
摘要
Abstract
Objective To examine an in vivo method for the differentiation of induced pluripotent stem cells (iPSCs) into thymic epithelial cells (TECs) in mice. Methods Green fluorescent protein-expressing iPS cells, derived from C57BL/6 mice, were injected into blastocysts from ICR mice. Chimeric blastocysts were then transferred into uteri of E2.5 pseudopregnant mice. Chimeric mouse could be identified by coat color 10 days after birth. The chimeric thymus was transplanted under the renal capsule of BALB/c nude mice. The spleen was cut out from the thymus-transplanted nude mice and the cells were dispersed and analyzed by a flow cytometer 4 weeks after transplantation. Results Chimeras were born 17 days after embryo transfer and 13 live-born chimeras were obtained. The contribution of iPSC-derived cells in the chimeras ranged from 5% to at most 90%. Typical thymic epithelium structure consisted of green fluorescent protein-expressing cells in chimera. The iPSCs-derived thymic epithelial cells could support the generation of new T cells. Conclusion The results indicate that mouse iPS cells can differentiate in vivo towards normally functioning TECs.关键词
iPS细胞/囊胚/显微注射/胸腺/嵌合体Key words
Induced pluripotent stem cells/Blastocyst/Microinjection/Thymus/Chimera引用本文复制引用
吴翠玲,郭雯铃,梁惠,石明,张玉明..体内诱导小鼠诱导性多能干细胞向胸腺上皮细胞分化的方法[J].实用医学杂志,2016,32(12):1916-1919,4.基金项目
国家自然科学基金（编号81270632，81302544）；广东省高等学校优秀青年教师培养计划（编号YQ2015082）；广东医学院人才引进人员科研启动项目 （）
