实用医学杂志2016，Vol.32Issue(12)：1934-1937,4.DOI:10.3969/j.issn.1006-5725.2016.12.012
下调Slit／Robo通路抑制兔血管成形术后血管再狭窄
The study of inhibit the Slit/Robo pathway on vascular restenosis after angioplasty in rabbits
摘要
Abstract
Objective To study the effect and mechanism of down-regulating Silt2/Robo 1 signaling pathway on rabbit iliac artery after angioplasty restenosis. Methods The 30 male New Zealand white rabbits were divided randomly into 3 groups , namely the blank group , the control group , and the experimental group , 10 rabbits in each group. Hign-fat feeding , the rabbits were produced endothelial denudation of iliac artery stenosis model. Another 4 weeks of feeding , percutaneous balloon angioplasty was performed. Then R5 antibody was injected into the abdominal cavity. After 4 weeks of feeding ,angiography again. The results of angiography was analysied by image workstation. The concentrations of Slit2 and Robo1 was detected by ELISA. The iliac artery tissue examined by HE staining. Results The rabbit iliac artery after angioplasty restenosis animal model was set up successfully. Compared with the control group and the experimental group , the serum concentration of Slit2 and Robo1 were significantly higher (P < 0.01) than the blank group. But in the experimental group, the Slit2 and Robo1 serum concentrations were significantly lower than those in the control group (P < 0.05) after R5 antibody intervention. The area ratio stenosis and diameter stenosis rate of iliac artery were reduced that confirmed by angiography. Conclusion The expression of Slit2/Robo1 was significantly higher in the rabbit model of vascular restenosis. R5 antibody can effectively inhibit the expression of Slit2/Robo1. Down regulation of Slit2/Robo1 signaling pathway in the treatment of restenosis after angioplasty in rabbits.关键词
Slit2/Robo1/R5抗体/血管成形术/再狭窄Key words
Slit2/Robo1/R5antibody/angioplasty/restenosis引用本文复制引用
曾智桓,张仁丹,赵艳群,郑坚奕,周万兴,张卫,朱桂平,李博维..下调Slit／Robo通路抑制兔血管成形术后血管再狭窄[J].实用医学杂志,2016,32(12):1934-1937,4.基金项目
广东省医学科研基金项目（编号A2014359）；广州市越秀区科信局基金项目 （）
