现代妇产科进展2016，Vol.25Issue(7)：11-14,4.DOI:10.13283/j.cnki.xdfckjz.2016.07.003
HPV16E6E7癌蛋白通过TWEAK/Fn14信号途径对细胞增殖和凋亡的影响
The effect of HPV16 E6E7 oncoproteins on cell proliferation and apoptosis via TWEAK/Fn14 signal pathway
摘要
Abstract
Objective:To investigate the effect of HPV16 E6 E7 oncoproteins on TWEAK/Fn14 signal pathway, proliferation and apoptosis. Methods:Recombinant retrovirus expressing HPV16 E6E7 was prepared and infect primary keratinocytes. Fn14 expression was determined using FACS. RANTES,MCP-1,IP10 and TRAF2 expressions were measured using real-time RT-PCR and ELISA assays. The activity of Ras GTPase was detected using biochemi-cal method. Cell apoptosis and proliferation were detected using TUNEL and MTT assay. Re-sults:The results showed that Fn14 was highly expressed upon infection and accompanied by an increase in RANTES,MCP-1,IP10 and TRAF2 expression levels,as well as Ras GTPase activi-ty. Moreover,keratinocytes infected by the Recombinant retrovirus expressing HPV16 E6E7 in-fected keratinocytes weakened apoptotic response to TNF-α stimuli compared with the control without HPV16 E6E7 expression. Significant increase in proliferation but not apoptosis was seen in E6E7 positive keratinocytes. Conclusions:HPV16 E6E7 expression activates TWEAK/Fn14 signal pathway,and up-regulates Ras and TRAF-2,leading to cell proliferation,not apoptosis, suggesting that it is another pathogenic mechanism of high-risk HPV infection.关键词
人乳头瘤病毒/凋亡肿瘤坏死因子样弱诱导因子Key words
Human papillomavirus/Tumor necrosis factorlike weak inducer of apoptosis分类
医药卫生引用本文复制引用
张广平,刘梦琼,胡波..HPV16E6E7癌蛋白通过TWEAK/Fn14信号途径对细胞增殖和凋亡的影响[J].现代妇产科进展,2016,25(7):11-14,4.基金项目
广东省自然科学基金(No:2014A030313095) （No:2014A030313095）
海南省自然科学基金资助项目(No:811177) （No:811177）
