现代妇产科进展2016，Vol.25Issue(7)：503-506,510,5.DOI:10.13283/j.cnki.xdfckjz.2016.07.006
人卵巢癌H08910细胞系中肿瘤干细胞样细胞通过MMP促进血管生成拟态的形成
Tumor stem-cell-like cells from human ovarian cancer cell line HO8910 and its relation to vasculogenic mimicry
摘要
Abstract
Objective:To investigate the relationship between VM( vascularization mim-icry) and tumor stem-cell-like cells from human ovarian cancer cells line HO8910. Methods:(1) The maternal HO8910 cells were cultured in serum-free stem cell medium by continuous passage. (2) The expression of tumor stem cells marker CD133 in the 1st,3rd,5th,7th passa-ges suspension cells were tested by Flow cytom etry. Flat colony formation test in vitro and the tumor formation test in vivo of nude mice were applied to identify the characteristic of tumor stem-cell-like cells in the 7th suspension cells. (3) Three-dimension cell culture was used to observe the ability of VM formation in the 7th suspension cells and maternal HO8910 cells. The expression levels of Metalloproteinases-2 ( MMP-2 ) and Metalloproteinases-9 ( MMP-9 ) in both of them were detected by real-time PCR(RT-PCR) method. Results:(1)The maternal HO8910 cells could continuous passages under the condition of serum-free medium, the suspension spheres became larger and larger with the passages. (2)The results of flow cytom etry illustrated that CD133 expression levels in the 1st,3rd,5th and 7th generation of suspension cells in-creased(0. 23%,31. 74%,79. 25% and 88. 18%). The colony-forming efficiency of the 7th generation suspension cells[(45. 67±1. 53)%]were significantly higher than those of the ma-ternal HO8910 cells[(11. 00±2. 00)%](t=23. 859,P<0. 00). The results of tumor formation in nude mice revealed that the rate of tumor formation in the 7th generation suspension cells(6/6 ) were significantly higher than maternal cells ( 0/6 ) . ( 3 ) Compared with maternal HO8910 cells,the 7th generation suspension cells took shorter time to form VM and more intensive tubu-lar structure in the condition of tree-dimensional cultivation. RT-PCR test showed the expression levels of MMP-2 and MMP-9 gene in the 7 th generation suspension cells were higher than those of the maternal cells(t=-4. 544,P=0. 045;t=-4. 019,P=0. 013). Conlusion:Tumor stem-cell-like cells could be isolated from the maternal HO8910 cells by serum-free suspension cul-ture and had ability to form VM. We speculate that tumor stem-cell-like cells may contribute to grow and transfer of ovarian cancer through influence the formation of VM.关键词
血管生成拟态/基质金属蛋白酶类/肿瘤干细胞/卵巢肿瘤/微循环Key words
Vasculogenic mimicry/Matrix metalloproteinases/Neoplastic stem cells/Ovarian neoplasms/Microcirculation分类
医药卫生引用本文复制引用
耿松珊,吴小华..人卵巢癌H08910细胞系中肿瘤干细胞样细胞通过MMP促进血管生成拟态的形成[J].现代妇产科进展,2016,25(7):503-506,510,5.基金项目
河北省政府资助临床医学优秀人才培养和基础课题研究项目计划(No:361001) （No:361001）
