现代妇产科进展2016，Vol.25Issue(7)：515-517,522,4.DOI:10.13283/j.cnki.xdfckjz.2016.07.009
NK-1受体拮抗剂对Ishikawa细胞抑制作用的研究
Inhibitory effect of NK-1 receptor antagonists on Ishikawa cells
摘要
Abstract
Objective:To investigate the effects of NK-1 receptor antagonist ( L-33 , 060) on proliferation,apoptosis,cell cycle and invasion of human endometrial adenocarcinoma Ishikawa cells. Methods:Human endometrial adenocarcinoma Ishikawa cells were cultured in vitro and treated with different concerration of L-33,060 for 48h respectively. MTT assay and flow cytometry were used to measure the effects of L-33,060 on cell proliferation and cell cycle. The activity of invasion was analyzed byTranswell assay. Results:L-33,060 inhibited cell prolif-eration of cultured Ishikawa cells (P<0. 05). L-33,060 blocked the cycle in G0 ~G1 period and increased the rates of cell apoptosis ( P<0 . 05 ) . The activity of invasion was inhitited by L-33,060(P<0. 05). All the effects were in a dose dependent manner. Conclusion:The NK-1 re-ceptor antagonist could inhibit cell proliferation,block cell cycles,induce cell apoptosis and ar-rest invasion. It would become a new therapies for endmetrial carcinoma.关键词
神经激肽1/子宫内膜癌/细胞凋亡/增殖/侵袭Key words
Neurokinin-1/Endometrial carcinoma/Apoptosis/Proliferation/Invasion分类
医药卫生引用本文复制引用
马静,赵雯红,袁世发,黄丽珍,张佳佳,程建新..NK-1受体拮抗剂对Ishikawa细胞抑制作用的研究[J].现代妇产科进展,2016,25(7):515-517,522,4.基金项目
河北省卫生和计划生育委员会医学科学研究重点课题(No:20150337) （No:20150337）
