中国组织工程研究2016，Vol.20Issue(28)：4203-4209,7.DOI:10.3969/j.issn.2095-4344.2016.28.015
视网膜干细胞移植对青光眼视网膜神经节细胞的保护
Protective effect of retinal stem cell transplantation on retinal ganglion cells in glaucoma
顾志敏 1周利晓 1齐若1
作者信息
- 1. 郑州大学第五附属医院眼科，河南省郑州市 450052
- 折叠
摘要
Abstract
BACKGROUND:Stem cel transplantation is a new method for blinding eye disease. But there is a lack of research about the protective effect of retinal stem cel transplantation on retinal ganglion cel s in glaucoma. OBJECTIVE:To explore the protective effect of retinal stem cel transplantation on retinal ganglion cel s of rats with glaucoma. METHODS:Forty-five Sprague-Dawley rats were randomly divided into three groups (n=15 per group) including control, model and retinal stem cel transplantation groups. Rat models of glaucoma were prepared in the latter two groups, and at 7 days after modeling, rats in the three groups were given intravitreal injection of 1 mL retinal stem cel s (5x106 cel s), the same amount of PBS, and no treatment, respectively. Subsequently, relative indicators were detected at 2 weeks after transplantation. RESULTS AND CONCLUSION:The expressions of brain-derived neurotrophic factor and insulin-like growth factor I protein as wel as the number of retinal ganglion cel s were the highest in the control group, fol owed by the retinal stem cel transplantation group model group, and the lowest in the model group (P<0.05). The number of apoptotic retinal ganglion cel s in model group was significantly higher than that of control group (P<0.05), and which in the retinal stem cel transplantation group was significantly lower than that in the model group (P<0.05), but higher than that in the control group (P<0.05). These results suggest that retinal stem cel transplantation for rat glaucoma can exert a protective effect on retinal ganglion cel s.关键词
干细胞/移植/视网膜/青光眼/大鼠/神经节细胞/保护作用分类
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顾志敏,周利晓,齐若..视网膜干细胞移植对青光眼视网膜神经节细胞的保护[J].中国组织工程研究,2016,20(28):4203-4209,7.
