中国组织工程研究2016，Vol.20Issue(29)：4349-4354,6.DOI:10.3969/j.issn.2095-4344.2016.29.013
间充质干细胞条件培养液对正常成纤维细胞及瘢痕成纤维细胞转化生长因子β产生和信号通路的影响
Effects of mesenchymal stem cells conditioned media on the transforming growth factor-beta signaling pathway in normal and hypertrophic scar fibroblasts
摘要
Abstract
BACKGROUND:Numerous studies have shown that mesenchymal stem cel s (MSCs) can effectively attenuate the fibrosis of damaged heart, lung and kidney by secreting various bioactive factors. OBJECTIVE:To evaluate the anti-fibrotic therapeutic potential of bone marrow MSCs conditioned media in vitro. METHODS:Normal fibroblasts and hypertrophic scar fibroblasts were treated with bone marrow MSCs conditioned media, then transforming growth factor-βand col agen production were analyzed by ELISA, and mRNA expression level of Smad7 and hydroxyproline content were detected by RT-PCR and colorimetry, respectively. RESULTS AND CONCLUSION:Bone marrow MSCs conditioned media significantly inhibited the production of both transforming growth factor-βand col agen in hypertrophic scar fibroblasts (P<0. 01), and up-regulated the mRNA expression level of Smad7 (P<0. 01), a major inhibitory regulator in the SMAD family. However, the normal fibroblasts were scarcely influenced by bone marrow MSCs conditioned media. These findings indicate that bone marrow MSCs conditioned media is considered a promising candidate for the treatment of hypertrophic scars, which may provide new theoretical supports to reduce cutaneous scarring.关键词
组织构建/组织工程/干细胞/间质干细胞/瘢痕/成纤维细胞/胶原/细胞增殖/羟脯氨酸分类
医药卫生引用本文复制引用
武艳,袁晓环,杨岚,赵孝金,李厚忠,王莹,金红..间充质干细胞条件培养液对正常成纤维细胞及瘢痕成纤维细胞转化生长因子β产生和信号通路的影响[J].中国组织工程研究,2016,20(29):4349-4354,6.基金项目
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015109) （UNPYSCT-2015109）
