现代医药卫生2016，Vol.32Issue(13)：1964-1966,3.DOI:10.3969/j.issn.1009-5519.2016.13.004
兔VX2肿瘤组织与正常组织微血管构筑形态研究
Study on microvasculature morphology from rabbit VX2 tumor tissue and normal tissue
摘要
Abstract
Objective To observe the morphological characteristics of microvasculature in rabbit VX2 muscle tumor and normal muscular tissue. Methods The VX2 muscle tumor model was established by the canalled tissue mass embedding method. The electron microscopy and microvascular casting techniques were used to observe the microvascular morphologic characteristics of normal muscular and muscle tumor. Results (1)The VX2 muscle tumor model rabbit was successfully established by the canalled tissue mass embedding method with a success rate of 100%.(2)The microvascular inner diameter of normal muscular tis-sue observed by the electron microscopy was more uniform ,the thickness of basement membrane was uniform with intact intima , the endothelial cells and perithelial cells were tightly adhered with the basement membrane. However ,the microvascular diameter in tumor tissue was uneven,the vessel lumen was mostly expanded with cystic change,thin and uneven basement membrane,loose attachment between endothelial cells and basement membrane ,and partial separation. (3)The microvascular casting directly showed the three-dimensional microvascular features of the normal muscle and tumor tissue. The microvascular density in normal muscle tissue was relatively sparse with uniform distribution and natural course like branches ,whereas the microvessels in the tu-mor tissue were intensive with structural distortion and ununiform distribution ,the majority of courses showed tortuous with snake-like and distributed unevenly,the part showed the cystic dilation,the part was tortuous and narrow,or the both coexisted and anas-tomosed into complex vascular network and vascular mass. Conclusion The morphological characteristics of microvasculature from rabbit VX2 muscle tumor tissue and normal muscle tissue have a significant difference.关键词
肿瘤,肌组织/肿瘤,实验性/肿瘤/病理学/组织包埋/毛细血管/超微结构/微血管构筑/兔Key words
Neoplasms,muscle tissue/Neoplasms,experimental/Neoplasms/pathology/Tissue embedding/Capillaries/ultrastructure/Microvascular architecture/Rabbits引用本文复制引用
黄泽君,李锐,孙建森,丁俊..兔VX2肿瘤组织与正常组织微血管构筑形态研究[J].现代医药卫生,2016,32(13):1964-1966,3.基金项目
国家自然科学基金资助项目（30770563）。 （）
