现代医药卫生2016，Vol.32Issue(13)：1967-1969,1972,4.DOI:10.3969/j.issn.1009-5519.2016.13.005
FSTL1及MMP-2在子痫前期孕妇胎盘组织及滋养细胞中的表达及相关性研究
Study on expression and correlation of FSTL1 and MMP-2 in placenta tissue and trophoblasts in preeclampsia pregnant women
摘要
Abstract
Objective To detect the expression and correlation of follistatin-like protein 1(FSTL1) and matrix metallo-proteinase-2(MMP-2) in placenta tissue and trophoblasts in preeclampsia pregnant women. Methods Thirty-five preeclampsia pregnant women and 40 normal full term pregnant women undergoing cesarean section in the obstetric department of the First Af-filiated Hospital of Chongqing Medical University from January 2014 to September 2015 were taken as the research subjects. Western blotting was adopted to detect the expression of FSTL1 and MMP-2 protein in placental tissues;the expressions of FSTL1 and MMP-2 in human early pregnancy extrachorionic trophoblastic cell lines HTR8/SVneo by normal culture and hypoxia/re-oxy-genation(H/R)treatment were detected by adopting immunofluorescence assay;the enzyme-linked immunosorbent assay(ELISA) was used to examine the levels of FSTL1 in serum in the two groups. The correlation between FSTL1 and MMP-2 expression levels with preeclampsia was analyzed. Results The expression level of FSTL1 in placenta tissues of preeclampsia patients was signifi-cantly increased compared with placenta tissue in full term pregnancy ,while the expression level of MMP-2 was significantly re-duced,and the differences were statistically significant(P<0.05). The expression level of FSTL1 protein in the cells of H/R treatment was significantly increased compared with that in the normal culture group ,while the expression level of MMP-2 was significantly reduced,and the differences were statistically significant(P<0.05);the positioning of FSTL1 was primarily located in cytoplasm of HTR8/SVneo. The serum FSTL1 level in the full term pregnant women group was significantly lower than that in the preeclampsia pregnant women,the difference was statistically significant(P<0.01). Conclusion The abnormal changes of FSTL1 and MMP-2 expression in placental tissues and serum may contribute to the pathogenesis of preeclampsia ,the two may have synergistic effect. Studying the interaction of FSTL1 and MMP-2 may provide a new idea for the research of preeclampsia pathogenesis.关键词
先兆子痫/胎盘/滋养层/卵泡抑素/基质金属蛋白酶2/氧化性应激/孕妇Key words
Pre-eclampsia/Placenta/Trophoblasts/Follistatin/Matrix metalloproteinase 2/Oxidative stress/Pregnant women引用本文复制引用
马红柳,饶海英,漆洪波,罗欣..FSTL1及MMP-2在子痫前期孕妇胎盘组织及滋养细胞中的表达及相关性研究[J].现代医药卫生,2016,32(13):1967-1969,1972,4.基金项目
国家自然科学基金资助项目（81300509、81471472）。 （）
