现代医药卫生2016，Vol.32Issue(13)：1973-1975,1978,4.DOI:10.3969/j.issn.1009-5519.2016.13.007
HMGB1表达水平对实验性结肠炎小鼠Th1/Tc1细胞反应的影响
Effects of HMGB1 expression level on Th1//Tc1 cellular response in experimental colitis mice
摘要
Abstract
Objective To investigate the expression level of high-mobility group box 1 protein (HMGB1) on helper T1/cytotoxic T cell 1(Th1/Tc1) in experimental colitis mice. Methods The BALB/c mice were randomly divided into ethanol control group,model group and ethyl pyruvate(EP) intervention group. The colitis model in the latter two groups was induced by the ene-ma of 2,4,6-trinitro-benzene sulfonic acid(TNBS). The disease activity index(DAI) score was daily evaluated. Mice were killed on 7 d after model construction. The colonic tissue pathological damage situation was observed. The flow cytometry was applied to examine the percentages of Th1//Tc1 cells in spleen as well as in mesenteric lymph nodes (MLN). The blood and colonic tissue ex-pression levels of HMGB1 and IFN-γwere measured by ELISA. Results The DAI and microscopic histopathological damage score in the model group were significantly increased compared with the ethanol control group (P<0.01);meanwhile the levels of HMGB1 and IFN-γin peripheral blood and colonic tissue and the percentages of Th1//Tc1 cells in spleen and MLN were signifi-cantly higher than those in the ethanol control group,the differences were statistically significant(P<0.01). After 1 week EP inter-vention,the HMGB1 level in serum and colonic tissue in the EP intervention group was significantly decreased,the DAI score was declined and microscopic histopathologic damage was obviously alleviated,the differences were statistically significant(P<0.05);with the percentage decrease of Th1/Tci cells in spleen and MLN,serum and colonic tissue IFN-γexpression was decreased,the difference was statistically significant(P<0.01). Conclusion HMGB1 may serve as the endogenous immunologic stimulant to in-duce and promote Th1/Tc1 cell hyperreaction,thus involves in pathogenesis of experimental colitis mice.关键词
结肠炎/HMGB1蛋白质/Th1细胞/T淋巴细胞,细胞毒性/干扰素Ⅱ型/三硝基苯磺酸/小鼠Key words
Colitis/HMGB1 protein/Th1 cells/T-lymphocytes,cytotoxic/Interferon typeⅡ/Trinitroben-zenesulfonic acid/Mice引用本文复制引用
郭向华,黎华辉,周联,罗霞,典灵辉,郭润华..HMGB1表达水平对实验性结肠炎小鼠Th1/Tc1细胞反应的影响[J].现代医药卫生,2016,32(13):1973-1975,1978,4.基金项目
中央财政支持地方高校发展专项资金资助项目[276（2014）]；广东省科技计划项目（2014A020212457）；广东省中医药局科研基金资助项目（20132209）；广东医科大学博士启动基金资助项目（2XB13094）。 （）
