现代医药卫生2016，Vol.32Issue(14)：2117-2119,2122,4.DOI:10.3969/j.issn.1009-5519.2016.14.001
β-葡聚糖对巨噬细胞源性泡沫细胞胆固醇逆转运的影响及机制
Effects ofβ-glucan on reverse cholesterol transport of macrophage-derived foam cells and its mechanism
摘要
Abstract
Objective To explore the effects ofβ-glucan on the reverse cholesterol transport of macrophage-derived foam cells and its relative mechanism. Methods Oxidized low-density lipoprotein(oxLDL) induced RAW264.7 macrophages were adopted as the foam cells model for giving theβ-glucan intervention. The Oil-red O staining and enzymatic colorimetry were em-ployed to examine the changes of intracellular total cholesterol and cholesteryl ester;Western blotting and real-time PCR were used to detect the effect of β-glucan on the expression on ATP-binding cassette transporter protein A1(ABCA1) and mRNA in macrophages. The obtained data were statistically analyzed. The protein synthesis inhibitors cycloheximide (CHX) processed cell 4,8,12 h,thus,deter minated ABCA1 stability. Results The inhibiting rate ofβ-glucan on macrophage′lipid uptake was(90.0± 1.8)%,total cholesterol and cholesteryl ester levels had statistical difference between the oxLDL group and the co-in cu bation groups of oxLDL and 5.0,10.0μg/mLβ-glucan(P<0.05);the expression of ABCA1 and mRNA had statistical differences between the 5.0,10.0μg/mLβ-glucan groups and the control group (P<0.01 or 0.05).β-glucan (10.0μg/mL) and CHX common treatment group in cell incubation 4,8,12 h,ABCA1 lower level less than CHX treatment group,the difference was statistically significant (P<0.05). Conclusion β-glucan promotes the intracellular cholesterol discharge and finally inhibits the formation of macrophage-derived foam cells by enhancing ABCA1 expression and protein stability.关键词
β葡聚糖类/巨噬细胞/胆固醇/泡沫细胞/胆固醇酯/动脉粥样硬化Key words
Beta-glucans/Macrophages/Cholesterol/Foam cells/Cholesterol esters/Atherosclerosis引用本文复制引用
李秀英,叶云..β-葡聚糖对巨噬细胞源性泡沫细胞胆固醇逆转运的影响及机制[J].现代医药卫生,2016,32(14):2117-2119,2122,4.基金项目
国家青年自然科学基金项目（81500357）；西南医科大学附属医院课题项目（15044）；西南医科大学课题项目（2015-03-10）。 （）
