现代医药卫生2016，Vol.32Issue(14)：2143-2145,3.DOI:10.3969/j.issn.1009-5519.2016.14.010
不同稀释液对检测血清乳酸脱氢酶的影响
Influence of different dilution solutions on serum LDH detection
方伟祯 1何健 1蔡振华 2陈梅 1叶远羊3
作者信息
- 1. 中山大学孙逸仙纪念医院检验科，广东广州510120
- 2. 广州中医药大学第一附属医院检验科，广东广州510405
- 3. 广州医科大学金域检验学院，广东广州510182
- 折叠
摘要
Abstract
Objective To investigate the influence of different dilution solutions on serum LDH detection by comparing the changes of serum LDH after specimen dilution. Methods The AU5800 automatic biochemical analyzer was used to detect the LDH concentrations before and after specimen dilution. The detection results of the same high LDH specimen were compared between the original concentration and concentrations after dilution by 3 kinds of dilution solutions of deionized water ,normal saline and inactivated serum. The degree of regression coefficient (RC) close to 1 was compared by drawing the regression curve. Results When 2 times of dilutions,RC of deionized water(RCbw)=1.038 4,RC of normal saline(RCbs)=1.043 4,RC of inactivated serum(RCbi)=1.023 4;when 5 times of dilutions,RCbw=1.152 9,RCbs=1.144 4,RCbi=1.142 0;when 10 times of dilutions,RCbw=1.107 8, RCbs=1.112 4,RCbi=0.995 8. Conclusion In the comparison of 2,5,10 of 3-dilution gradients,RCbi is most close to 1 compared with RCbs and RCbw,indicating that inactivated serum is the best dilution solution among these 3 kinds of dilution solution.关键词
乳酸脱氢酶/血清/去离子水/生理盐水/稀释液Key words
L-lactate dehydrogenase/Serm/Deionized water/Normal saline/Diluent引用本文复制引用
方伟祯,何健,蔡振华,陈梅,叶远羊..不同稀释液对检测血清乳酸脱氢酶的影响[J].现代医药卫生,2016,32(14):2143-2145,3.
