摘要
Abstract
Objective The release of HMGB1 of Tetrandrine n on titanium particles stimulate blood mononuclear cell (PBMC) was approached,to supply documentation for prevention artificial knee joint aseptic loosening. Methods The cells were divided into 4 groups ,cells in group 1 were added with nothing, while cells in group 2,3,and 4 were added with titanium particles(0.1%),and the cells in group 3,4 were added with Tetrandrine (1 g/ml,10 g/ml).The contents of HMGB1 protein and HMGB1Mrna of the 4 groups were respectively detected through enzyme linked immunosorbent assay (ELISA)and polymerase chain reaction (PCR) after culturing for 48 hours. Results ①Effect of titanium particles suspension concentration on HMGB1expression: There were statistical differences in the contents of HMGB1 protein and HMGB1 m RNA of macrophages among the 2 groups ( =2600.42,<0.01; =516.55,<0.01).② Effect of titanium particles and Tetrandrine on the expression of HMGB1 protein and HMGB1 m RNA : There were statistical differences in the contents of HMGB1 protein and HMGB1 m RNA of macrophages among the 2 group and 3/4 group ( =17.73,<0.01; =723.45,<0.01); There were statistical differences among the 1 group and 3/4 group ( =2153.03,<0.01; F=1177.804,<0.01).Conclusion Wear particles from joint prosthesis can up-regulate the expression of HMGB1 ,which leads to bone absorption and osteolysis. Tetrandrine could realsed the expression of HMGB1, which would be used to patients for prevention artificial knee joint aseptic loosening.关键词
汉防己甲素/钛颗粒/单个核细胞/高迁移率族蛋白-1Key words
Tetrandrine/titanium particles/PBMC/HMGB1
