医药导报2016，Vol.35Issue(8)：819-822,4.DOI:10.3870/j.issn.1004-0781.2016.08.004
资木瓜总苷对大鼠嗜碱性白血病-2H3肥大细胞脱颗粒的作用∗
Effects of Total Saponins of Chaenomeles speciosa on Degranulation Ratio of RBL-2H3 Mast Cells
摘要
Abstract
Objective To study the effects of total saponins of Chaenomeles speciosa on release of β-hexosaminidase from rat basophilic leukemia-2H3 ( RBL-2H3 ) mast cells. Methods After rat RBL-2H3 mast cells were prepared, total saponins of Chaenomeles speciosa and the RBL-2H3 mast cells were co-cultured.The toxic effects of total saponins of Chaenomeles speciosa on mast cells were detected by MTT method, β-hexosaminidase release rate was measured by fluorescence quantitative spectrophotometric method, and cell supernatants of tumor necrosis factor-α( TNF-α) release were detected by ELISA. Results After total saponins of Chaenomeles speciosa were co-cultured with RBL-2H3 mast cells with different antigen stimulation, β-hexosaminidase release rates and the levels of TNF-α of mast cells significantly decreased compared with the control group. Conclusion Total saponins of Chaenomeles speciosa inhibit the degranulation of RBL-2H3 mast cells in a dose dependent manner, which provids basis for studying mechanism of inhibiting allergic reactions.关键词
资木瓜总苷/嗜碱性白血病-2H3,大鼠/β-己糖胺酶/肿瘤坏死因子Key words
Total saponins of Chaenomeles speciosa/Basophilic leukemia-2H3,rat/β-hexosaminidase/Tumor necrosis factor分类
医药卫生引用本文复制引用
贾云莉,李世刚,柳蔚,喻玲玲,曾小威..资木瓜总苷对大鼠嗜碱性白血病-2H3肥大细胞脱颗粒的作用∗[J].医药导报,2016,35(8):819-822,4.基金项目
国家自然科学基金资助项目(81274166) （81274166）
