医药导报2016，Vol.35Issue(8)：835-839,5.DOI:10.3870/j.issn.1004-0781.2016.08.008
芹菜素对人NCI-H1650细胞的增殖抑制及诱导凋亡作用∗
Effect of Apigenin on Inhibiting Proliferation and Apoptosis of Human NCI-H1650 Cell Line
摘要
Abstract
Objective To determine the effect of apigenin on inducing apoptosis and inhibiting proliferation of non-small cell lung cancer cell line NCI-H1650. Methods NCI-H1650 cell line was cultured routinely in vitro, with blank control group and different concentrations of apigenin (10, 20, 40, 80 μmol�L-1). The blank control group was RPMI-1640 solution without apigenin. Cell proliferation was detected by MTT. Hoechst 33258 was applied to observe morphological changes of the apoptotic cells after treatment of different concentrations of apigenin. Flow cytometry AnnexinV-FITC/PI double staining method was used to determine cell apoptosis rate. The expression levels of apoptosis-related signaling molecules Bax and Bcl-2 protein were performed by Western blotting. Results MTT showed that apigenin inhibited proliferation of NCI-H1650 cell line in a concentration-and time-dependent manner (P<0.01).Hoechst 33258 nuclear staining showed typical characteristics of apoptosis in certain concentration-dependent manner, such as nuclear condensation and appearance of apoptotic bodies. In addition, the results of flow cytometry staining indicated that the apoptotic rate of NCI-H1650 cells cultured with blank control group and different concentrations of apigenin (10, 20, 40, 80 μmol�L-1) for 48 h was (5.00±0.33)%, (18.05±4.67)%, (21.48± 1.95)%, (43.24±1.11)%, (66.23±3.65)%, respectively (P<0.01).Western blotting results showed that the expression levels of Bax increased with increasing of the drug concentration, but Bcl-2 decreased with increasing of the drug concentration. Conclusion Apigenin can inhibit the proliferation and induce apoptosis of NCI-H1650 cells in non-small cell lung cancer. Mechanisms may be related to increase of the expression of apoptosis related protein Bax and decrease of the expression of anti apoptosis protein Bcl-2.关键词
芹菜素/癌,肺,非小细胞/增殖抑制/凋亡Key words
Apigenin/Cancer, lung, non-small/Inhibiting proliferation/Apoptosis分类
医药卫生引用本文复制引用
潘晓彤,杨周萍,刘欢,潘雪刁..芹菜素对人NCI-H1650细胞的增殖抑制及诱导凋亡作用∗[J].医药导报,2016,35(8):835-839,5.基金项目
国家级大学生创新创业训练计划项目(20131057313010) （20131057313010）
