医药导报2016，Vol.35Issue(8)：861-866,6.DOI:10.3870/j.issn.1004-0781.2016.08.015
高效液相-蒸发光散射检测器测定黄芪中4种皂苷类成分
Assay of Four Saponins in Astragali Radix by HPLC-ELSD
蔡海霞 1赵红卫 1秦玉花 1柴东燕1
作者信息
- 1. 河南省人民医院药学部，郑州 450003
- 折叠
摘要
Abstract
Objective To study the feasibility of high performance liquid chromatography-evaporative light scattering detector ( HPLC-ELSD) in quantitative analysis of multi-components ( QAMS) by single marker. Methods Four saponins in Astragali Radix were simultaneously determined by HPLC-ELSD using external standard method, and malonylastragaloside I served as internal standard. The relative correction factors between internal standard and astragaloside Ⅰ, astragaloside Ⅱ and astragaloside Ⅳ were calculated, and the stability was investigated. Results Astragaloside Ⅳ in Astragali Radix was little, while malonylastragalosideⅠand AstragalosideⅠwere abundant.The relative correction factors lacked stability, so ELSD could not be used in QAMS. Conclusion HPLC-ELSD can precisely determine contents of four saponins in Astragali Radix. The detector needs to be further studied when the components have poor ultraviolet absorption such as saponins by QAMS.关键词
黄芪/皂苷/一测多评/检测器,高效液相-蒸发光散射Key words
Astragali Radix/Saponins/Single marker/Detector, high performance liquid chromatography-evaporative light scattering分类
中医学引用本文复制引用
蔡海霞,赵红卫,秦玉花,柴东燕..高效液相-蒸发光散射检测器测定黄芪中4种皂苷类成分[J].医药导报,2016,35(8):861-866,6.
