中国病理生理杂志2016，Vol.32Issue(7)：1161-1166,6.DOI:10.3969/j.issn.1000-4718.2016.07.002
外源性硫化氢通过抑制JAK／STAT通路对抗高糖引起的人脐静脉内皮细胞损伤
Exogenous hydrogen sulfide attenuates high glucose-induced injury by in-hibiting JAK/STAT pathway in human umbilical vein endothelial cells
摘要
Abstract
AIM: To explore whether exogenous hydrogen sulfide (H2S) depresses high glucose (HG)-in-duced injury by modulating the Janus kinase/signal transducer and activator of transcription ( JAK/STAT) pathway in hu-man umbilical vein endothelial cells (HUVECs).METHODS:The protein levels of JAK2, STAT3 and cleaved caspase-3 were determined by Western blot.The cell viability was measured by CCK-8 assay.Mitochondrial membrane potential ( MMP) was detected by rhodamine 123 staining followed by photofluorography.The intracellular level of reactive oxygen species (ROS) was analyzed by DCFH-DA staining followed by photofluorography.The activity of superoxide dismutase (SOD) was also measured.RESULTS:Pretreatment of the HUVECs with 400 μmol/L NaHS (a donor of H2S) for 30 min prior to exposure to 40 mmol/L glucose ( HG) markedly attenuated HG-induced upregulation of the phosphorylation of JAK2 and STAT3.Pretreatment with 400μmol/L NaHS for 30 min or with 20μmol/L AG490 (inhibitor of the JAK/STAT pathway) for 30 min attenuated the injury of HUVECs induced by HG, as indicated by the increases in cell viability and SOD activity, and decreases in the protein level of cleaved caspase-3, ROS generation and dissipation of MMP.CONCLU-SION:Exogenous H2 S protects HUVECs against HG-induced injury by inhibiting JAK/STAT pathway.关键词
硫化氢/JAK/STAT通路/高糖/人脐静脉内皮细胞Key words
Hydrogen sulfide/JAK/STAT pathway/High glucose/Human umbilical vein endothelial cells分类
医药卫生引用本文复制引用
林佳琼,陈景福,廖静秋,林凯,邓海鸥,吴冬波,吴文..外源性硫化氢通过抑制JAK／STAT通路对抗高糖引起的人脐静脉内皮细胞损伤[J].中国病理生理杂志,2016,32(7):1161-1166,6.基金项目
国家自然科学基金资助项目（No．81450062）；广东省自然科学基金资助项目 （）
