中国动物检疫2016，Vol.33Issue(7)：78-81,94,5.DOI:10.3969/j.issn.1005-944X.2016.07.024
非洲猪瘟病毒RPA等温检测方法的建立
Rapid and Sensitive Detection of African Swine Fever Virus by Recombinase Polymerase Ampliifcation
王建昌 1王金凤 1刘立兵 1孙晓霞 1袁万哲2
作者信息
- 1. 河北出入境检验检疫局技术中心，河北石家庄 050051
- 2. 河北农业大学动物医学院，河北保定 071001
- 折叠
摘要
Abstract
To develop an convenient and rapid molecular diagnostic method for the African swine fever virus(ASFV), the recombinase polymerase amplification(RPA)was developed using primers specific for the conserved region of the ASFV P72 gene. The RPA reaction was performed successfully at 38℃for 30 min in a water bath. By using recombinant plasmid DNA carrying the P72 gene as template.The results showed that the detection limit of RPA was 102 copies of plasmid DNA,which was the same as the real-time PCR recommended by OIE,and it was 10 times more sensitive than it. RPA could not amplify templates of FMDV,CSFV,PRRSV,PRV or PCV-2,demonstrating high specificity.The ASFV RPA developed in the study is simple,rapid,cost-effective and reliable,which can be an novel and reliable tool for the control of ASF.关键词
非洲猪瘟病毒/分子检测/等温扩增/聚合酶重组酶扩增/快速Key words
African swine fever virus/molecular diagnosis/isothermal amplification/recombinase polymerase amplification/rapid分类
农业科技引用本文复制引用
王建昌,王金凤,刘立兵,孙晓霞,袁万哲..非洲猪瘟病毒RPA等温检测方法的建立[J].中国动物检疫,2016,33(7):78-81,94,5.
