中国感染控制杂志2016，Vol.15Issue(7)：447-451,5.DOI:10.3969/j.issn.1671-9638.2016.07.002
长沙地区产金属β-内酰胺酶铜绿假单胞菌的分子流行病学特征
Molecular epidemiological characteristics of metallo-β-lactamase-producing Pseudomonas aeruginosa from Changsha
摘要
Abstract
Objective To investigate the genotypes and epidemic of metallo-β-lactamase-(MBL )-producing Pseudomonas aeruginosa (P .aeruginosa)in Changsha.Methods P .aeruginosa isolated from seven comprehensive hospitals in Changsha were collected and performed identification and antimicrobial susceptibility testing,pheno-types of MBL were detected with EDTA-disk synergy test and E-test,genotypes were determined by polymerase chain reaction (PCR),homology analysis were conducted by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR).Results Preliminary screening by EDTA-disk synergy test and E-test showed that only 10 of 81 iso-lates were strong positive;PCR result showed that 18 isolates were positive for MBL,11 of which were IMP-9-type MBL,1 was IMP-1-type,and 6 were VIM-2-type.SIM,SPM,GIM,and NDM-1-types were not found.ERIC-PCR showed that 12 strains of IMP-producing P .aeruginosa has multiple types,6 VIM-2-producing strains were of the same type.Conclusion IMP-9 and VIM-2 are main genotypes in P .aeruginosa in Changsha.关键词
铜绿假单胞菌/金属β-内酰胺酶/金属酶/基因型/肠杆菌科基因间重复序列聚合酶链反应Key words
Pseudomonas aeruginosa/metallo-β-lactamase/metalloenzyme/genotype/enterobacterial repetitive intergenic consensus-polymerase chain reaction分类
预防医学引用本文复制引用
邬靖敏,邹明祥,李军..长沙地区产金属β-内酰胺酶铜绿假单胞菌的分子流行病学特征[J].中国感染控制杂志,2016,15(7):447-451,5.基金项目
湖南省发改委（湘发改高技［2012］1493号；湘发改投资［2014］658号）；湖南省自然科学基金 （）
