局解手术学杂志2016，Vol.25Issue(7)：473-477,5.DOI:10.11659/jjssx.06E016009
家蝇β-葡萄糖苷酶在Ⅲ龄幼虫消化系统以外器官的表达
Expression of musca domestica β-glucosidase in the organs besides digestive system of Ⅲ instar larvae
摘要
Abstract
Objective To study whether the organs besides digestive system of musca domestica Ⅲ instar larvae have the capability of produceing musca β-glucosidase.Methods Tissues of malpighian tubules,trachea,epiploon and body wall of musca domestica Ⅲ instar lar-vae were dissected under anatomic microscope,and the expression of β-glucosidase gene in these dissected tissues were detected by reverse transcription PCR.And the tissue localization of β-glucosidase mRNA was further identified by in situ hybridization.Moreover,anti-cellulase was used to determinate the tissue distribution with immunohistochemical staining.The relative mRNA expression levels of musca domesticaβ-glucosidase gene in these organs were tested by real-time quantitative PCR.Results The reverse transcription PCR showed that the ampli-fication products of β-glucosidase gene were observed in tissues of malpighian tubules,trachea and body wall.β-glucosidase mRNA was shown in the epithelium cells of malpighian tubules,trachea and body wall by in situ hybridization,and it was almost the same in the results of im-munohistochemical staining.The real-time quantitative PCR showed that the relative expression quantity of β-glucosidase gene in malpighian tubules and body wall were higher than that in foregut,while it was lower in itrachea than that in foregut.And it was of statistical difference in gene expression level of β-glucosidase among these organs (P <0.05).Conclusion Malpighian tubules,trachea and body wall of musca domestica Ⅲ instar larvae have the function of secreting β-glucosidase.Combining with the characteristics of secreting β-glucosidase in most organs of digestive system,it may provide a new biological method for the prevention and treatment of human diseases transmitted by musca domestica with the use of taget gene β-glucosidase.关键词
β-葡萄糖苷酶/家蝇/表达/Ⅲ龄幼虫Key words
β-glucosidase/musca domestica/expression/Ⅲ instar larvae分类
基础医学引用本文复制引用
胡蓉,张姝,黄悦,苏敏,李红,国果,付萍,吴建伟..家蝇β-葡萄糖苷酶在Ⅲ龄幼虫消化系统以外器官的表达[J].局解手术学杂志,2016,25(7):473-477,5.基金项目
国家科技支撑计划（2011BAC06B12）；国家自然科学基金 （）
