中国康复理论与实践2016，Vol.22Issue(7)：745-749,5.DOI:10.3969/j.issn.1006-9771.2016.07.001
自噬对血管性痴呆大鼠海马CA1区生长相关蛋白-43及微管相关蛋白-2表达的影响
Effects of Autophagy on Expression of Growth-associated Protein-43 and Microtubule Associated Protein-2 in CA1 Area of Hippocampus of Vascular Dementia Rats
摘要
Abstract
Objective To observe the effects of autophagy on the expression of synaptic plasticity related protein, growth-associated pro-tein-43 (GAP-43) and microtubule associated protein-2 (MAP-2), in CA1 area of hippocampus of vascular dementia rats. Methods Nine-ty-six healthy male Sprague-Dawley rats were randomly divided into sham group, vascular dementia model group (VD group), autophagy in-hibitor 3-methyl adenine preconditioning group (3-MA group) and autophagy agonist rapamycin preconditioning group (Rap group). Each group was divided randomly into subgroups of one week, two weeks, four weeks and eight weeks after modeling, six rats in each group. The vascular dementia rat model was established with modified Pulsineli's four-vessel occlusion. The expression of GAP-43 and MAP-2 in CA1 area of hippocampus were detected with immunohistochemistry. Results Compared with the sham group, the expression of GAP-43 protein increased, and the expression of MAP-2 protein decreased at every time point in VD group (P<0.01). Compared with VD group, the expres-sion of both GAP-43 and MAP-2 increased in 3-MA group (P<0.05), and decreased in Rap group (P<0.05). Conclusion Autophagy may in-hibit the expression of synaptic plasticity related protein, GAP-43 and MAP-2, in CA1 area of hippocampus in vascular dementia rats, indi-cating inhibition of autophagy may promote synaptic remodeling.关键词
血管性痴呆/自噬/生长相关蛋白-43/微管相关蛋白-2/突触可塑性/大鼠Key words
vascular dementia/autophagy/growth-associated protein-43/microtubule associated protein-2/synaptic plasticity/rats分类
医药卫生引用本文复制引用
张文彦,刘金霞,刘斌,邓春颖,张晋霞,马原源,毛文静,李世英,吕超男..自噬对血管性痴呆大鼠海马CA1区生长相关蛋白-43及微管相关蛋白-2表达的影响[J].中国康复理论与实践,2016,22(7):745-749,5.基金项目
1.河北省高等学校科学技术研究重点项目(No.ZH2012046)；2.河北省级重大医学科研课题(No.zd2013087)。 （No.ZH2012046）
