中国康复理论与实践2016，Vol.22Issue(7)：750-753,4.DOI:10.3969/j.issn.1006-9771.2016.07.002
复方脑肽节苷脂注射液激活线粒体自噬改善脑缺血再灌注损伤
Effect of Compound Porcine Cerebroside and Ganglioside Injection on Cerebral Ischemia-reperfusion Injury in Rats
摘要
Abstract
Objective To investigate the neuroprotective effect and possible mechanism of Compound Porcine Cerebroside and Ganglio-side Injection (CPCGI) on cerebral ischemia-reperfusion injury in rats. Methods Healthy adult male Sprague-Dawley rats were divided into sham group (n=10), model group (n=10), CPCGI low dosage group (n=10) and high dosage group (n=10), and control group (Ginkgo biloba extract, n=10). All the rats was subjected to middle cerebral artery occlusion (MCAO) for two hours and reperfusion except sham group, and received treatment for fourteen days once reperfusion started. They were tested with modified Neurological Severity Score one, three, seven and fourteen days after MCAO, and adhesive-removal test and beam-walking test fourteen days after MCAO. The expression of Beclin1, PINK1 and Parkin were detected with Western blotting. Results Compared with the model group, the Neurological Severity Score reduced (P<0.05) and the time crossing the beam reduced (P<0.01) in all the medical groups fourteen days after MCAO, and the time removing the adhesive paper reduced in the CPCGI groups (P<0.01). The expression of Beclin1 and Parkin decreased and the PINK1 level increased in the model group (P<0.01), and it was reversed in all the CPCGI groups (P<0.05). Conclusion CPCGI could relieve the cerebral ischemia-re-perfusion injury in rats through the regulation in mitophagy.关键词
脑缺血再灌注损伤/复方脑肽节苷脂注射液/线粒体自噬/大鼠Key words
cerebral ischemia-reperfusion injury/Compound Porcine Cerebroside and Ganglioside Injection/mitophagy/rats分类
医药卫生引用本文复制引用
王明洋,冯璐,范姝婕,郑吉,李冬梅,杨楠,左萍萍,刘雁勇..复方脑肽节苷脂注射液激活线粒体自噬改善脑缺血再灌注损伤[J].中国康复理论与实践,2016,22(7):750-753,4.基金项目
北京协和青年基金(No.3332015113)。 （No.3332015113）
