中国康复理论与实践2016，Vol.22Issue(7)：759-764,6.DOI:10.3969/j.issn.1006-9771.2016.07.004
有氧运动预处理对全脑缺血再灌注大鼠再生相关因子的影响
Effect of Aerobic Exercise Preconditioning on Growth-associated Protein-43 and Nogo-A in Rats after Cerebral Ischemia/Reperfusion
摘要
Abstract
Objective To observe the effect of aerobic exercise preconditioning on growth-associated protein-43 (GAP-43) and Nogo-A in rats after cerebral ischemia/reperfusion. Methods Sprague-Dawley rats were equally divided into sham group (n=40), cerebral ischemia/reperfusion group (n=40) and aerobic exercise preconditioning group (n=40), and global cerebral ischemia model was formed with modified four-vessel occlusion. The rats was sacrificed six hours, one day, three days and seven days after ischemia, respectively. The hippocampus neural cells were observed in five rats with HE staining and immunohistochemistry of GAP-43 and Nogo-A, and the other five rats were test-ed with RT-PCR of GAP-43 and Nogo-A. Results Compared with those in the cerebral ischemia/reperfusion group, the apoptotic neurons and expression of GAP-43 significantly increased all the time points in the aerobic exercise preconditioning group (P<0.01), while the ex-pression of Nogo-A decreased (P<0.01). Conclusion Aerobic exercise preconditioning can promote the regeneration of neuronal cells and axon after cerebral ischemia/reperfusion injury, which is related to the regulation of GAP-43 and Nogo-A.关键词
脑缺血再灌注/有氧运动/生长相关蛋白-43/Nogo-A/大鼠Key words
cerebral ischemia/reperfusion/aerobic exercise/growth-associated protein-43/Nogo-A/rats分类
医药卫生引用本文复制引用
孙竹梅,李建民,王国立,赵雅宁,陈长香,赵旭,王静..有氧运动预处理对全脑缺血再灌注大鼠再生相关因子的影响[J].中国康复理论与实践,2016,22(7):759-764,6.基金项目
1.河北省卫生厅课题(No.ZD2010106)；2.唐山市科技计划项目(No.14130220B)。 （No.ZD2010106）
