中国康复理论与实践2016，Vol.22Issue(7)：774-778,5.DOI:10.3969/j.issn.1006-9771.2016.07.007
褪黑素对脊髓损伤大鼠突触可塑性的作用
Effects of Melatonin on Synaptic Plasticity after Spinal Cord Injury in Rats
摘要
Abstract
Objective To observe the effects of melatonin on synaptic plasticity impaired by spinal cord injury in rats. Methods A total of 54 female Sprague-Dawley rats were divided into sham group (n=18), control group (n=18) and melatonin group (n=18). Spinal cord inju-ry model was established with modified Allen's method at T10 (10 g from 25 mm height). The number of neurons and the expression of the Nissl body were detected with immunofluorescence and Nissl staining. The expression of neurofilament-200 (NF-200), brain-derived neuro-trophic factors (BDNF), Synapsin I and growth-associated protein-43 (GAP-43) was detected with Western blotting. Results Seven days af-ter injury, the number of motoneurons, the expression of Nissl body in motoneurons, and the expression of BDNF, Synapsin I and GAP-43 decreased in the control group compared with those in the sham group, and they increased in the melatonin group compared with those in the control group. Conclusion Melatonin can repair the impaired synaptic plasticity, which might promote the functional recovery after spi-nal cord injury.关键词
脊髓损伤/突触可塑性/褪黑素/大鼠Key words
spinal cord injury/synaptic plasticity/melatonin/rats分类
医药卫生引用本文复制引用
荆瀛黎,刘小野,白帆,董浩,陈惠..褪黑素对脊髓损伤大鼠突触可塑性的作用[J].中国康复理论与实践,2016,22(7):774-778,5.基金项目
1.北京脑重大疾病研究院科研促进项目(No.PXM2014_014226_000016)11421311；2.神经损伤与康复北京市重点实验室2015年度科技创新基地培育与发展专项项目(No.Z151100001615055)；3.中央级公益性科研院所基本科研业务费专项资金项目(No.2015CZ-12)。 （No.PXM2014_014226_000016）
