中国康复理论与实践2016，Vol.22Issue(7)：784-788,5.DOI:10.3969/j.issn.1006-9771.2016.07.009
运动时不同吸氧浓度对慢性阻塞性肺疾病大鼠活性氧类物质产生的影响
Effect of Oxygen Concentration during Exercise on Reactive Oxygen Species Production in Chronic Obstructive Pulmonary Disease Rats
摘要
Abstract
Objective To investigate the effect of various concentration of inhaled oxygen during exercise on reactive oxygen species (ROS) production in chronic obstructive pulmonary disease (COPD) rats and the possible mechanism. Methods Eighty COPD Wistar rats were divided into low oxygen (LO, n=20), normal oxygen (NO, n=20) and inhaled oxygen (IO, n=20) exercising groups, which ran on tread-mill in the conditions of 13.6%, 21%, 25%oxygen, respectively, and non-exercising normal oxygen group (C, n=20), which stood on still treadmill in the condition of 21%oxygen. Their apoptosis percentage of neutrophils and ROS content were measured with flow cytometry, glutathione (GSH) in lung with immunohistochemistry and cytochrome C oxidase IV (COXIV) in skeletal muscle with Western blotting, as one and four weeks of exercising. Results For one week of exercise, the ROS was more in LO and NO groups than in C group (P<0.05), while the neutrophils apoptosis percentage was less (P<0.05);and there was no difference among C, LO, NO, IO groups in expression of GSH and COXIV (P>0.05). For four weeks of exercise, the ROS was more in LO group but less in NO and IO groups than in C group (P<0.05);while the expression of GSH and COXIV increased in NO and IO groups compared with those in C group (P<0.05). Conclusion A long term exercise in non-hypoxic state may inhibit ROS production for COPD patients, by promoting neutrophils apoptosis and antioxidant expression.关键词
慢性阻塞性肺疾病/运动/氧浓度/吸氧/活性氧类物质/大鼠Key words
chronic obstructive pulmonary disease/exercise/oxygen concentration/inhaled oxygen/reactive oxygen species/rats分类
医药卫生引用本文复制引用
刘衡,温宝伶,王晓龙..运动时不同吸氧浓度对慢性阻塞性肺疾病大鼠活性氧类物质产生的影响[J].中国康复理论与实践,2016,22(7):784-788,5.基金项目
中央高校基本科研业务费(No.106112015CDJXY250002)。 （No.106112015CDJXY250002）
