中国临床药理学杂志2016，Vol.32Issue(13)：1198-1201,4.DOI:10.13699/j.cnki.1001-6821.2016.13.014
丁酸钠对小鼠结肠炎性相关细胞因子平衡的影响
Effects of sodium butyrate on the balance of inflammation -related cytokines in colitic mice
摘要
Abstract
Objective To observe the effect of butyrate on the balance of T helper cell ( Th) one , Th2 and Th17 -type cytokines in the colon of dextran sulphate sodium salt ( DSS ) -induced colitic mice. Methods Eighty male Balb/c mice was randomly divided into four groups as below:normal group , model group , blank group and test group.A murine model of DSS-induced colitis was induced among mo-del group, blank group and test group.After treatment of DSS, butyrate (100 mg・ kg -1・ d -1 , 0.2 mL) was used in test group by intragastric administration once a day for 7 d.The weight , survival rate and length of colon were evaluated.The production of Th1 ( interleukin -4, IL-6, IL-10 ) , Th2[ tumor necrosis factor-α( TNF-α);inferon-γ( IFN-γ) ,IL-17 ] and Th17-type cytokines ( IL-17 ) was detected by enzyme -linked immunosorbent assay .The pathological change of colon was observed by light microscope with HE staining . The pathological grade was evaluated . Results Compared with normal group , the weight of mice and the length of colon in model group were significantly de-creased ( P<0.05 ).The expression of cytokines was increased .Compared with blank group , the weight of mice and the length of colon in test group were increased ( P<0.05 ) and the survival rate rised ( test group:70%, blank group:50%) . Compared with blank group , Th1, Th2 and Th17 -type cytokines in test group were deceased:(367.89 ±29.83 ) vs ( 693.49 ±228.77 ) pg・ mL-1 , ( 256.29 ±34.18 ) vs ( 704.49 ±179.00 ) pg・ mL-1 , (392.44 ±28.70 ) vs ( 992.34 ±159.93 ) pg・ mL-1 , ( 361.81 ±63.39 ) vs ( 816.31 ±204.00 ) pg・ mL-1 , (302.43 ±42.59 ) vs ( 527.58 ±152.27 ) pg・ mL-1 , ( 250.10 ±36.15 ) vs ( 400.16 ±118.28 ) pg・ mL -1 ( all P<0.05).It is near to those in normal group.The ratios of IL-4, IL-6, IL-10, TNF-α, IFN-γand IL-17 between test and normal group were 1.94, 1.51, 1.62, 1.13, 1.34 and 1.31, respectively.The pathological grade all the mice in model group got to the level above II .The percentage of mice in blank group and test group that below the grade of Ⅱwere 38.5% and 71.0%, which meant the pathological change in test group was better than that in blank group.Conclusion Butyrate could promote remission of colitis in mice by keeping the balance of Th 1, Th2 and Th17-type cytokines.关键词
丁酸钠/白细胞介素-4/肿瘤坏死因子-α/结肠炎小鼠Key words
butyrate/interleukin-4/tumor necrosis factor-α/colitic mice分类
医药卫生引用本文复制引用
林雪,王帆,冯爵荣,赵秋,陶文惠,李瑾..丁酸钠对小鼠结肠炎性相关细胞因子平衡的影响[J].中国临床药理学杂志,2016,32(13):1198-1201,4.基金项目
国家青年自然科学基金资助项目 （）
