中国临床药理学杂志2016，Vol.32Issue(14)：1272-1275,4.DOI:10.13699/j.cnki.1001-6821.2016.14.007
研究新疆不同民族之间糖尿病患者和健康受试者的血浆氨基酸含量的差异
Study on the variance of amino acid contents in plasma between different ethnic with diabetic patients in Xinjiang
摘要
Abstract
Objective To analysis the change of amino acid metabolism in plasma in Xinjiang Han , Uygur , Kazakh and other ethnic with diabetic patients.Methods All the subjects were divided into 6 group: Han-normal group, Uygur -normal group , the other -normal group;Han -diabetic group, Uygur -diabetic group, the other -diabetic group, in which plasma were deproteinization by capronitrile . Reversed -phase HPLC with pre-column derivatization was used to determine plasma le-vels of amino acids.The amino -acid was derivatized with 6 -ami-noquinolyl-N-hydroxysuccinimicly carbamate (AQC).Waters-e2695 operating system was used.Column for Shimadzu Shim -VP, ODS (4.6 mm ×250 mm,5 μm).A certain percentage of methanol -acetonitrile-water and sodium acetate as the mobile phase gradient elution ( pH 6.5 ) was used. The 17 kinds of free amino acid content in plasma were measured by fluorescence detector .Results In diabetic group and the corresponding normal groups , leucine, isoleu-cine content was significantly lower than that of the corresponding national normal group in Han , Uygur and the other ethnic.While the contents of threonine , alanine, cysteine, tyrosine, valine, lysine, methionine, phenylalanine, lysine amino acid were significantly higher than those of the corresponding national normal groups ( P<0.05 ).The content of arginine in plasma of the patients with diabetes was significantly lower than that in the normal group in Han and Uygur.The serine of plasma in Han-diabetic group was significantly higher than that in the normal group .The levels of aspartic acid content in Uygur -diabetic group was significantly lower than that of the normal group ( P<0.05).The level of serine and threonine were increased in Uygur ehnic diabetic group compared with Han ethnic , and glycine , argi-nine significantly reduced than compared with the Kazakhs ( P<0.05).Conclusion Compared with their own normal group, varying degrees of disorder of plasma amino acid metabolism had found in Xinjiang different ethnic diabetes groups , and then the difference of plasma amino acid have their own characteristics when compared with different ethnic .关键词
高效液相色谱法/血浆/糖尿病/氨基酸/民族Key words
HPLC/plasma/type 2 diabetes/amino acid/ethnic分类
医药卫生引用本文复制引用
何新苗,张雨洁,毛新民,巴吐尔,马晓丽..研究新疆不同民族之间糖尿病患者和健康受试者的血浆氨基酸含量的差异[J].中国临床药理学杂志,2016,32(14):1272-1275,4.基金项目
国家自然科学基金资助项目 （）
