中国临床药理学杂志2016，Vol.32Issue(14)：1307-1310,4.DOI:10.13699/j.cnki.1001-6821.2016.14.017
通窍活血汤对大鼠缺血性脑卒中神经递质的影响
Effect of Tongqiao Huoxue Decoction on neurotransmitter in rats with cerebral ischemic stroke
摘要
Abstract
Objective To study effects of Tongqiao Huoxue Decoction ( THD) on ischemic stroke nerve injury in rats .Methods The 50 SPF level healthy male Wistar rats was selected using a random number table method and divided into sham group , model group , low and high doses of two test groups , and control group , ten rats in each group .The focal cerebral ischemia ( MCAO) model were prepared.Low and high doses of two test groups were given THD 0.9 , 1.8 g・ mL-1 in accordance with 5 mL・ kg -1 orally, control group rats were given 36 mg・ mL-1 piracetam tablets aqueous solution 5 mL・ kg -1 orally; sham group and model group were fed with 5 mL・ kg -1 saline.All rats were fed twice a day , a total of 20 d.The neuromotor function , changes level of serum nitric monoxide ( NO) , plasma endothelin-1 ( ET-1 ) and the ratio between the NO/ET-1 levels and brain glutamic acid ( Glu ) , aspartic acid ( Asp) were observed in rats of five groups.Results After administration of 20 d, the motor function scores in low and high doses of two test groups with (6.15 ±0.08), (6.58 ±0.08) and control group with (6.08 ±0.15), were signifi-cantly higher in model group with (5.07 ±0.12 ) ( P <0.05 ) .The ET -1 level in low and high doses of two test groups with (69.72 ±5.76),(57.32 ±6.14)pg・ mL-1 and control group with (70.12 ±5.86)pg・ mL-1,were sig-nificantly lower in model group with(94.22 ±6.38)pg・ mL-1(P<0.05).The serum NO level in low and high doses of two test groups with (51.82 ±2.36), (65.72 ±2.44)μmol・ L-1 and control group with (52.68 ±2.55)μmol・ L-1, was increased signi-ficantly than in model group with (40.36 ±2.48)μmol・ L-1(P<0.05).The levels of Glu in low and high doses of two test groups with ( 6.35 ±1.18 ) , ( 5.08 ±1.06 ) μmol・ L-1 and control group with (6.68 ±0.94 )μmol・ L-1 were decreased significantly than in model group with (9.58 ±1.34)μmol・ L-1 (P<0.05). The levels of Asp in low and high doses of two test groups with (10.16 ±0.12),(8.24 ±0.28)μmol・ L-1 and control group with (10.04 ±0.18)μmol・ L-1 was decreased significantly than in model group with (12.42 ±0.25)μmol・ L-1 ( P<0.05).Conclusion High dose THD can obviously improve nerve motor function of the ischemic cerebral apoplexy rats, increase the serum and plasma NO and ET 1 level , while lower Glu , Asp levels in brain tissue .关键词
缺血性脑卒中/通窍活血法/神经运动功能/神经递质Key words
cerebral ischemic stroke/Tongqiao Huoxue method/nerve function/neurotransmitter分类
中医学引用本文复制引用
江颖..通窍活血汤对大鼠缺血性脑卒中神经递质的影响[J].中国临床药理学杂志,2016,32(14):1307-1310,4.基金项目
广西省自然科学基金资助项目 （）
