中国临床药理学杂志2016，Vol.32Issue(14)：1314-1317,4.DOI:10.13699/j.cnki.1001-6821.2016.14.019
羟苯磺酸钙对糖尿病大鼠视网膜细胞凋亡的影响
Effect of calcium dobesilate on the apoptosis of retinal cells in diabetic rats
摘要
Abstract
Objective To explore the effect of calcium dobesilate on the apoptosis of retinal cells in diabetic rats .Methods Thirty-six Wistar rats were randomly into three groups:normal group ( n =12 ) , model group(n=12), test group(n=12).The rat in model and test groups of diabetes was given with high fat diet for 8 weeks, and then was made by intraperitoneal injection of streptozotocin ( STZ ) .After successfully es-tablished the diatetes animal model , the rats in test group was lavaged with 150 mg・ kg -1・ d-1 calcium dobesilate continuous 8 weeks, the rats in normal and model group were given same amount of normal saline . Neuronal apoptosis in retina was detected by TdT -mediated dUTP nick end labeling ( TUNEL ) . The expression of B -cell lymphoma -2 ( Bcl-2 ) , Bcl -2 assaciated X protein ( Bax ) and p53 protein and mRNA were assayed by Western blot and reverse transcription -polymer-ase chain reaction.The activity of cysteinyl aspartate specific proteinase 3 ( Caspase 3) was measured by spectrophotometry .Results Compared with normal group , apoptotic index of retina , the expression of Bax , p53 protein and mRNA , the activity of Caspase 3 was increased ( P<0.01 ) , the expression of Bcl -2 protein and mRNA was decreased in model group ( P<0.01 ) . The expression of Bax , Bcl -2 and p53 mRNA was ( 0.20 ±0.03 ) vs ( 1.00 ±0.09 ) , ( 1.39 ±0.10 ) vs (0.21 ±0.02);(0.28 ±0.05) vs (0.88 ±0.08), in normal group and model group, respectively.Compared with model group,apoptotic index of retina (12.40 ±1.24), the expression of Bax (0.57 ±0.05) and p53 (0.35 ±0.03) protein, the expression of Bax ( 0.56 ±0.05 ) and p53 ( 0.36 ±0.04 ) mRNA, the activity of Caspase 3 (1.72 ±0.17) in test group were all decreased.While the expression of Bcl -2 protein (0.63 ±0.06) and mRNA (0.74 ±0.04 ) were increased in test group ( all P<0.01 ) .Conclusion The calcium dobesilate inhibit neuronal apoptosis by regulation expression of cell apoptotic related protein .关键词
羟苯磺酸钙/糖尿病视网膜病变/细胞凋亡Key words
calcium dobesilate/diabetic retinopathy/cell apoptosis分类
医药卫生引用本文复制引用
法利,裴存文,金约西..羟苯磺酸钙对糖尿病大鼠视网膜细胞凋亡的影响[J].中国临床药理学杂志,2016,32(14):1314-1317,4.基金项目
温州市科技计划基金资助项目 （）
