中国临床药理学杂志2016，Vol.32Issue(14)：1318-1321,4.DOI:10.13699/j.cnki.1001-6821.2016.14.020
微小 RNA542－3p与结直肠癌细胞侵袭转移能力的相关性研究
Relationship between micro RNA542 -3 p and the invasion and metastasis for colorectal cancer cell
摘要
Abstract
Objective To evaluate the molecular mechanism of micro RNA542-3p (miR-542-3p) in the invasion and metastasis of colorec-tal cancer.Methods Forty paired colorectal cancer patients with cancer tissue and relative normal colorectal tissue , different metastasis ability colorectal cancer cell lines ( Lovo, LS1747, SW620, HT29, SW480 ) and normal colorectal epithelial cells were tested of the miR-542-3 p expression by real -time polymerase chain reaction.The ability of prolif-eration or migration for SW480 or HT29 cells were tested after transinfec-tion of miR-542-3p, mimics and inhibitor after 24 h.The physiologi-cal characteristics such as proiferation , migration of colon cancer cells was evaluated.The biological function of miR -542-3 p was evaluated in colorectal cancer cells.Results MiR-542-3p expression in cancer tissues was significantly lower than that in normal intestinal epithelium ( P<0.05 ) .MiR-542-3 p was higher expressed in normal colorectal epithelial cells compared to colorectal cancer cell lines ( HT29 , SW620 and Lovo , P<0.05 ) .MiR-542-3 p was higher expressed in the high metastasis cancer cell lines ( SW620 and Lovo ) compared to low metastasis cancer cells (P<0.05).After 24, 48, 72 and 96 h, the cell proliferation ability was no significant difference in inhibitor mir-542-3p inhibitor group, miR-542-3p mimics group, control group, mimics control group (P>0.05).After 24 h, the migration ability in mir -542-3p mimics group was significantly lower than that of mimics control group ( P<0.05 ) , and the migration ability in miR -542-3 p inhibitor group was significantly higher than that of inhibitor control group (P<0.05).Conclusion MiR-542-3p may play a role in inhibiting cell metastasis in colon cancer cells, which can be used as biomarker for colorectal cancer metastasis .关键词
结直肠癌/微小RNA542-3p/侵袭转移/细胞株Key words
colorectal cancer/micro RNA542-3 p/invasion and metastasis/cell line分类
医药卫生引用本文复制引用
姚坤厚,马咏萍,胡军红,孟继明..微小 RNA542－3p与结直肠癌细胞侵袭转移能力的相关性研究[J].中国临床药理学杂志,2016,32(14):1318-1321,4.基金项目
河南省科技攻关计划（国际科技合作）基金资助项目 （）
