中国临床药理学杂志2016，Vol.32Issue(14)：1322-1324,3.DOI:10.13699/j.cnki.1001-6821.2016.14.021
重组人血管内皮抑制素与白细胞介素－2对卵巢癌作用的实验研究
Experimental study of function of recombinant human endostatin and interleukin-2 in the treatment of ovarian cancer
摘要
Abstract
Objective To investigate the efficacy of recombinant human endostatin and interleukin -2 in the treatment of ovarian cancer . Methods Eighty nude mices with ovarian cancer were randomly divided into negative control group , positive control group and the test A , B group, each group was 20 cases.Negative control group had no special treatment.Positive control group was treated with intravenous injection of 10 mg・ kg -1 recombinant human endostatin injection , qd.Test A group was given intramuscular injection of interleukin -2 , 1.0 ×10 6 U・ d -1 , qd.Test B group was given stem cells of amniotic fluid transfected with interleukin-2 gene, intravenous injection of 0.2 mL microbubbles, qd. Four groups were treated for seven weeks .Tumor volume was mersured every 3 days, and the volume, weight, inhibition rate of all ovarian cancers’ tumor block were mersured after 5 d of last handling.Tumor cell apoptosis was mersured by UNEL detection .Results Tumor volume increased with the time for four groups , and the increasing trend of negative control group was significantly higher than that of positive control group and test A , B group ( P <0.05 ) , but the increasing trend of positive control group and test A , B group had no statistical difference (P>0.05).After treatment, the tumor weight of positive control group and test B group was significantly lower than that of negative control group and test A group [(878.3 ±30.5),(902.4 ±34.6)mg vs (1554.7 ±45.3),(1484.6 ±41.4) mg, P<0.05 ].The difference of the tumor weight between the positive control group and test B group was not statisti -cally significant ( P>0.05 ) , so was that between the negative control group and test A group .Tumor inhibition rates and number of apoptotic cells in positive control group and test B group were significantly higher than those of negative control group and test A group (P<0.05).The difference of the tumor inhibition rates and number of apoptotic cells between the positive control group and test B group was not statistically significant ( P>0.05 ) , so was that betwenthe negative control group and test A group .Conclusion Amniotic fluid stem cellstransfected with interleukin -2 gene can inhibit the growth of ovarian can-cer, and promote the apoptosis of tumor cells .关键词
羊水干细胞靶向治疗/白细胞介素-2基因/卵巢癌Key words
amniotic fluid stem cell targeted therapy/interleukin-cell -2/ovarian cancer分类
医药卫生引用本文复制引用
于燕,张晓娜,亓健,王晓燕,毕春晖..重组人血管内皮抑制素与白细胞介素－2对卵巢癌作用的实验研究[J].中国临床药理学杂志,2016,32(14):1322-1324,3.基金项目
山东省卫生厅一般计划基金资助项目 （）
