中国免疫学杂志2016，Vol.32Issue(7)：952-956,961,6.DOI:10.3969/j.issn.1000-484X.2016.07.004
PLTP在香烟诱导HBECs合成IL-8中的作用
Effect of PLTP on CSE-induced IL-8 production in human bronchial epithelial cells
摘要
Abstract
Objective:To investigate the effect of PLTP gene on CSE-induced IL-8 production in human bronchial epithelial cell line (HBECs). Methods:Wistar rats were exposed to air or cigarette smoke for 6 hours/day on 3 consecutive days,then the lungs were sectioned and examined. The number of total white blood cell and differential white blood cells in BALF were counted. The different concentrations of CSE co-cultured with HBECs for 24 hours. Cells growth was detected by MTT assay. Expression levels of PLTP mRNA and IL-8 mRNA were examined by RT-PCR,protein of PLTP was investigated by Western blot,and production of IL-8 ex-amined by ELISA. Results:The number of white blood cells in BALF was significantly increased compared with controls. Enhanced ex-pression level of PLTP and IL-8 were observed in CS-exposure group. Proliferation of HBECs tends to decrease at high concentrations of CSE(2. 0% CSE and 4. 0% CSE). The results suggested that the production of IL-8 induced by CSE in a time- and concentration-dependent manner,while the expression of PLTP induced by CSE in a dose-dependent manner. Furthermore,expression levels of IL-8 significantly increased after silence PLTP gene. Conclusion:PLTP siRNA could increase CSE-induced IL-8 production in HBECs.关键词
烟草烟雾提取物/人支气管上皮细胞/磷脂转运蛋白/白介素8Key words
Cigarette smoke extract/Human bronchial epithelial cells/HBECs/PLTP/IL-8分类
医药卫生引用本文复制引用
余秀英,黎友伦,巫凤苹,陈亚娟..PLTP在香烟诱导HBECs合成IL-8中的作用[J].中国免疫学杂志,2016,32(7):952-956,961,6.基金项目
本文为国家自然科学基金青年基金资助项目( No.81200009)、国家临床重点专科专项资助(卫办医政函[2012]649号)和重庆市卫生局中医药研究课题(ZY20132165)。 （ No.81200009）
