中国免疫学杂志2016，Vol.32Issue(7)：970-973,4.DOI:10.3969/j.issn.1000-484X.2016.07.008
低剂量纳曲酮对小鼠巨噬细胞的免疫调节作用及机理研究
Immuno-regulatory effects on murine macrophages by low dose naltrexone (LDW)
摘要
Abstract
Objective:The effect of naltrexone on function of murine peritoneal macrophage in vitro was studied in order to illuminate its immune activity futher. Methods:Peritoneal macrophages were divided in three groups:RPMI1640 blank control group, LPS positive control group and NTX treated group. Various phenotypic and functional indices were tested by MTS, flow cytometry technology,phagocytosis experiment and ELISA. Results: Compared with RPMI1640 group,at a LDN exhibits paradoxical properties;the expression of CD64 on surface increased while the expression of CD206 decreased in NTX group;the expression of tumour necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β)were increased. Conclusion:The results of experiment had proved that LDN could influence macrophage polarzation, regulate the inflamatory mediators production and affect the phagocytosis function of peritoneal macrophage.关键词
纳曲酮/巨噬细胞/表型/细胞因子/吞噬能力Key words
Naltrexone/Macrophage/Phenotype/Cytokine/Phagocytosis分类
基础医学引用本文复制引用
郭胜男,李岩,王晓楠,单风平..低剂量纳曲酮对小鼠巨噬细胞的免疫调节作用及机理研究[J].中国免疫学杂志,2016,32(7):970-973,4.基金项目
本文为辽宁省科技厅基金项目(No.2012-225014)。 （No.2012-225014）
