中国免疫学杂志2016，Vol.32Issue(7)：974-978,5.DOI:10.3969/j.issn.1000-484X.2016.07.009
微小RNA-146a上调cyclinD1表达诱导大鼠血管平滑肌细胞的增殖
MicroRNA-146 a promotes proliferation of rat vascular smooth muscle cells by up-regulating cyclin D1 expression
摘要
Abstract
Objective:To detect and verifica the gene profile difference of microRNA-146a (miR-146a) and its role in the pro-liferation of vascular smooth muscle cells (VSMCs) by gene chip technology. Methods: Artificially synthesized miR-146a mimics(50 nmol/L) ,miR-146 inhibitor ( 50 nmol/L ) , scramble ( 50 nmol/L ) and PBS were transfected into cultured primary rat VSMCs in vitro. After transfection,Real time PCR was used to measure the levels of miR-146a and the cell counting kit 8(CCK8) was employed to investigate the proliferation of VSMCs. The VSMCs interfered by miR-146a inhibitor or miR-146a control were examined by gene chips and the profile of gene were analyzed by bioinformatics technology to detect the different genes and signal transduction pathway. The changes in mRNAs and proteins were accessed separately by Real time PCR and Western blot. Results: Compared with sham and control VSMCs,miR-146a expression level was significantly decreased in treatment with miR-146a inhibitor(P<0. 01),as well as optical density(OD) was also shown remarkably down regulated simultaneously(P<0. 05). The investigation of gene profile revealed that the p53 signal pathway was up-regulated in VSMCs interfered by miR-146a. The mRNA and protein expression levels of p53, caspase3 and PTEN in p53 signal transduction pathway didn′t show significant differences(P>0. 05),however,the mRNA and protein expression levels of cyclin D1 significantly increased in treatment with miR-146a mimics VSMCs group and decreased in miR-146a inhibitor VSMCs group ( compared with sham VSMCs group, both P<0. 05 ) . Conclusion: Our data indicated that miR-146a may promote the proliferation of rat VSMCs by up-regulating cyclin D1 expression.关键词
血管平滑肌细胞/miR-146a/基因谱/P53/cyclin D1Key words
Vascular smooth muscle cell/miRNA-146a/Gene profile/p53/cyclin D1分类
临床医学引用本文复制引用
熊玮,骆瑜,董少红,李江华,廖碧红,庞新利,罗林杰..微小RNA-146a上调cyclinD1表达诱导大鼠血管平滑肌细胞的增殖[J].中国免疫学杂志,2016,32(7):974-978,5.基金项目
本文为深圳市卫计委资助课题(No.201505001)。 （No.201505001）
