中国免疫学杂志2016，Vol.32Issue(7)：983-987,5.DOI:10.3969/j.issn.1000-484X.2016.07.011
表达H1 N1亚型猪流感病毒三聚体HA重组慢病毒的免疫原性研究
Study on immunogenicity of recombinant lentivirus expressing trimeric HA of swine H1 N1 influenza virus
摘要
Abstract
Objective:To evaluate the protective immunity by vaccination of BALB/c mice with rLV-HA-GCN4,a recombinant lentivirus expressing the trimeric HA of swine H1N1 influenza virus. Methods:The female mice were randomly divided into rLV-HA-GCN4,rLV-HA,LV and PBS groups. Mice were primed with plasmid and boosted with lentivirus by the administration of intramuscular thigh injections at an interval of two weeks. At day 28 post-prime immunization,mice were inoculated intranasally with 100TCID50 of swine H1N1 influenza virus in a 50 μl volume. The immune levels were assessed by the T lymphocyte transformation test, flow cytometry,indirect ELISA and the indexes of spleen and lung. Results:The spleen lymphocyte transformation rate was 0. 3±0. 11 in the rLV-HA-GCN4 group at day 14 post-boost immunization, showing a statistical significance ( P<0. 01 ) compared to the PBS group. Meanwhile,rLV-HA-GCN4 could cause T lymphocyte response mainly based on the Th1-type CD4+ T cells. The IgG antibody titer reached to 1:8 000 at day 14 post-boost immunization and approximately 1:7 000 at day 14 post challenge. After challenge,the spleen and lung indexes of rLV-HA-GCN4 group were significantly lower than those of PBS group (P<0. 05). The body weight of rLV-HA-GCN4 group demonstrated a slight decrease before 3 days post challenge and then a gradual increase compared to the LV and PBS groups (P<0. 05). Conclusion:rLV-HA-GCN4 can effectively induce cellular and humoral immune response in BALB/c mice against swine H1N1 influenza virus.关键词
猪流感/H1N1亚型/三聚体HA/慢病毒载体/免疫原性Key words
Swine influenza/H1N1 subtype/Trimeric HA/Lentiviral vector/Immunogenicity分类
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邓颖琦,李俊峰,曲辉,唐雨博,孙艺学,李鑫,王伟利,丁壮,丛彦龙..表达H1 N1亚型猪流感病毒三聚体HA重组慢病毒的免疫原性研究[J].中国免疫学杂志,2016,32(7):983-987,5.基金项目
本文为国家质检总局科技计划项目(2014IK237)和吉林省科技厅重点科技攻关项目(20150204029NY)。 （2014IK237）
