中国免疫学杂志2016，Vol.32Issue(7)：988-991,4.DOI:10.3969/j.issn.1000-484X.2016.07.012
小柴胡汤对C6胶质瘤抑瘤作用的实验研究
Experimental study of effect of Xiao Chai Hu Tang on C6 glioma
摘要
Abstract
Objective:To investigate the inhibitory effect and mechanism of different doses of Xiao Chai Hu Tang on C6 glioma cells cultured in vitro. Methods:C6 glioma cells were inoculated in 96 holes,24 holes and 6 holes,each culture plate was divided into 4 groups:control group, low dose group, middle dose group and high dose group, when the cells covered the bottom of culture plate 80%-90%,began adding,cultured for 24 hours after the ter mination of training. Cell proliferation activity,cell viability,protein content and protein positive expression intensity of VEGF and ESM-1,cell apoptosis in early and late stage were detected by CCK-8,in vivo staining,ELISA, immunocytochemistry and flow cytometry. Results: CCK-8 assay showed that the growth of C6 glioma cells was inhibited by low,medium and high dose group;ELISA and immunocytochemistry showed that the expression of VEGF and ESM-1 was lower in the lower, middle and high dose groups, and the levels of protein expression and protein levels were decreased. The flow cytometry showed that the low dose of small radix,middle and high dose group could promote the cell apoptosis. Inverted microscope ob-servation showed that with the increase of dose,the number of cells increased gradually,and the number of dead cells increased,and all kinds of detection methods showed that the inhibition effect increased with dose and dose dependence. The difference between the two groups was statistically significant(P<0. 01). Conclusion:The growth of C6 glioma cells was significantly inhibited by Xiao Chai Hu Tang. It may play a role in inhibiting tumor growth by down regulating ESM-1 and VEGF protein level and promoting cell apoptosis.关键词
小柴胡汤/C6胶质瘤/CCK-8/ESM-1/VEGF/流式细胞仪Key words
Xiao Chai Hu Tang/C6 glioma/CCK-8/ESM-1/VEGF/Flow cytometry分类
医药卫生引用本文复制引用
于慧玲,麻春杰,韩雪梅,赵鹏伟,赵灵燕..小柴胡汤对C6胶质瘤抑瘤作用的实验研究[J].中国免疫学杂志,2016,32(7):988-991,4.基金项目
本文为内蒙古自治区科技计划项目(kjt14sf14)。 （kjt14sf14）
