中国普通外科杂志2016，Vol.25Issue(7)：991-997,7.DOI:10.3978/j.issn.1005-6947.2016.07.010
microRNA-616在肝癌中的表达及临床意义
Expression of microRNA-616 in hepatocellular carcinoma and its clinical significance
摘要
Abstract
Objective: To investigate the microRNA-616(miR-616) expression in hepatocellular carcinoma (HCC) and its clinical signiifcance. <br> Methods: The miR-616 expressions in specimens of HCC and adjacent non-tumorous tissue from 80 HCC patients, as well as in normal hepatic cells and different HCC cell lines were determined by real-time PCR. hTe relations of miR-616 expression with pathologic factors and prognosis of HCC patients were analyzed and the changes in invasion and metastatic abilities in HCC cells atfer miR-616 overexpression or inhibition were observed. <br> Results: The miR-616 expression in HCC tissue was significantly elevated compared with adjacent non-tumorous liver tissue, and further, in recurrent cases was signiifcantly higher than that in non-recurrent cases and in cases with metastasis was signiifcantly higher than that in cases without metastasis(allP<0.05). hTe miR-616 expressions were signiifcantly increased in all HCC cell lines compared with normal hepatic cells, which in highly invasive HCC cells were signiifcantly higher than those in lowly invasive HCC cells (allP<0.05). hTe miR-616 expression was signiifcantly associated with the presence or absence of portal vein tumor thrombus, Edmondson-Steiner grade and TNM stage of HCC patients (allP<0.05), and both overall survival and disease-free survival in HCC patients with high miR-616 expression were signiifcantly lower than those in patients with low miR-616 expression(bothP<0.05). The invasive and metastatic abilities in HCC cells were significantly increased after miR-616 overexpression and were signiifcantly reduced atfer miR-616 inhibition (allP<0.05). <br> Conclusion: MiR-616 expression is increased in HCC, and the high miR-616 expression level is closely associated with poor prognosis.关键词
癌,肝细胞/微RNAs/预后Key words
Carcinoma, Hepatocellular/MicroRNAs/Prognosis分类
医药卫生引用本文复制引用
张立,张迪,李孝彬,王炜,贺赛,李杰,刘阳,孙学军..microRNA-616在肝癌中的表达及临床意义[J].中国普通外科杂志,2016,25(7):991-997,7.基金项目
国家自然科学基金资助项目（81172362；81172359）；陕西省科学技术研究发展计划基金资助项目（2013K12-05-01）。 （）
