中国水稻科学2016，Vol.30Issue(4)：335-344,10.DOI:10.16819/j.1001-7216.2016.6006
过表达一个疣粒野生稻的Ricin_B 凝集素基因OmR40c1增强水稻耐盐性
Over-expression of OmR40c1,a Ricin_B Lectin from Oryza meyeriana L. Enhances Salt Tolerance in Transgenic Plants
摘要
Abstract
Salt stress is a major environmental factor limiting rice growth and productivity in saline soil.Planting tolerant line is still the most effective way in response to salt stress.OsR40c1 is an abscisic acid (ABA)and salt stress-induced gene.However,the function of OmR40c1 is poorly studied.Here,we cloned a gene from Oryza meyeriana which shared the same CDS with OmR40c1 but differed in 5′&3′UTR.Subcellular location analysis showed that OmR40c1 was located in cytoplasmic membrane and nuclear.OmR40c1 overexpressing transgenic rice was obtained by using the Agrobacterium-mediated gene transfer system.OmR40c1 might involve in seed germination.The germination rate of OmR40c1 transgenic rice was slightly affected by salt stress,while wild type decreased about 50% compared with their mocks.The seedlings of wild type hardly grown on the 1/2 MS medium with salt (174 mmol/L NaCl),the height of 1 0-day OmR40c1 transgenic rice seedling decreased more than a half.Green leaves and root of OmR40c1 and wild type rice were both seriously affected by salt stress.The concentration of Na+in OmR40c1 was about 1.38 times higher than that in wild type,together with a slight rise in the K+ concentration and the results suggest that OmR40c1 may act as a salt tolerance gene in enhancing rice salt tolerance especially at the adult stage.关键词
疣粒野生稻/OmR40c1/转基因/耐盐性Key words
Oryza meyeriana/OmR40c1/transgenic rice/salt tolerance分类
生物科学引用本文复制引用
陈贤,董岩,周洁,王栩鸣,严成其,陈剑平..过表达一个疣粒野生稻的Ricin_B 凝集素基因OmR40c1增强水稻耐盐性[J].中国水稻科学,2016,30(4):335-344,10.基金项目
国家基础研究计划资助项目(2014CB1603090) （2014CB1603090）
国家863计划资助项目(2014AA0A603-15) （2014AA0A603-15）
浙江省自然科学基金资助项目(LZ14C140001,LZ2013C1109) （LZ14C140001,LZ2013C1109）
