中国水稻科学2016，Vol.30Issue(4)：345-355,11.DOI:10.16819/j.1001-7216.2016.5184
水稻脆秆突变体bc16的鉴定和基因精细定位
Identification and Gene Fine Mapping of a Brittle Culm 16 (bc16)Mutant in Rice
摘要
Abstract
Brittle culm mutants are one of the important materials to study plant mechanical strength.A brittle culm mutant named as bc16 (brittle culm 16 )was isolated from Nipponbare through EMS (Ethyl Methane Sulphonate) treatment.Compared with wild type,bc16 featured fragile and easily broken stems,leaves,roots,lower plant height and filled grains per panicle,shorter panicle length and main root length.The components analysis of stem cell wall showed that the cellulose content in bc16 mutant was sharply decreased,while hemi-cellulose content was evidently in-creased and no significant difference had been found in the lignin content between bc16 mutant and wild type.The cell shape and arrangement of parenchyma cells in bc16 mutant became irregular and disordered.Moreover,the parenchyma cell walls and the secondary cell walls of sclerenchyma cells in bc16 mutant under the epidermis layer were thinner than those in wild type.The results of genetic analysis revealed that the mutant trait was controlled by a single recessive gene,which was mapped in a 66.6-kb region between InDel (insertion-deletion)markers 2-F and 2-H on chromosome 2 with 7 candidate genes.Among these genes,Os02g0738900 ,which is allelic to BC3 gene,was specially focused on. There was a T to A substitution at the very end of the 13th intron ofOs02g0738900 which was at the 5113th base loca-tion from initiation codon by sequencing,which led an splicing forward mutation in the process of transcription,and six nucleotides were cut into mRNA as a result,which finally cause the stop of translation.Additionally,in the mutant, there were significant decrease in the expression of bc16 gene in roots,culms and leaves by real-time PCR method.The bc16 might affect the synthesis of sclerenchyma secondary cell wall and parenchyma primary cells wall,which could af-fect the mechanical strength of rice stem.关键词
水稻/脆秆/基因定位/细胞壁合成Key words
rice/brittle culm/gene mapping/cell wall synthesis分类
生物科学引用本文复制引用
舒亚洲,曾冬冬,秦冉,金晓丽,郑希,石春海..水稻脆秆突变体bc16的鉴定和基因精细定位[J].中国水稻科学,2016,30(4):345-355,11.基金项目
国家科技支撑计划资助项目(2011BAD35B02) （2011BAD35B02）
浙江省科技厅水稻产业科技创新服务平台 （）
高等学校学科创新引智计划资助项目(Grant B14027) （Grant B14027）
浙江省重大科技攻关专项(2012C12901-2) （2012C12901-2）
教育部创新团队资助项目(IRT1185) （IRT1185）
