中国水稻科学2016，Vol.30Issue(4)：356-362,7.DOI:10.16819/j.1001-7216.2016.5193
水稻草矮病毒侵染的水稻根系差异蛋白鉴定
Identification of Differentially Expressed Proteins in Roots of Rice (Oryza sativa)Infected with Rice grassy stunt virus
摘要
Abstract
In order to find the proteins related to root maldevelopment caused by Rice grassy stunt virus (RGSV), elucidate the mechanism of root maldevelopment and better understand the molecular basis of interaction between RGSV and host rice,2D-DIGE coupled with MALDI-TOF-MS was used to screen and identify differentially expressed proteins between RGSV-infected and RGSV-uninfected rice roots.The acquired proteins were further analyzed using GoMiner programs and KEGG pathway database.The results showed that 56 protein spots (differential ratio>2)were differentially expressed,including 34 up-regulated and 22 down-regulated protein spots.Among them,27 differentially expressed protein spots which belonged to 25 proteins were identified,and these proteins were mainly involved in 14 biological processes,1 0 molecular functions and 1 2 KEGG pathways.关键词
水稻草矮病毒/水稻根系/差异表达蛋白/双向荧光差异凝胶电泳/基质辅助激光解析串联飞行时间质谱技术Key words
Rice grassy stunt virus/rice roots/differentially expressed proteins/2D-DIGE/MALDI-TOF-MS分类
农业科技引用本文复制引用
丁新伦,谢荔岩,张洁,吴祖建..水稻草矮病毒侵染的水稻根系差异蛋白鉴定[J].中国水稻科学,2016,30(4):356-362,7.基金项目
国家自然科学基金资助项目(31301640) （31301640）
国家科技支撑计划资助项目(2012BAD19B03) （2012BAD19B03）
