中国水稻科学2016，Vol.30Issue(4)：397-405,9.DOI:10.16819/j.1001-7216.2016.6011
水稻转录因子基因OsPHR3在磷素利用过程中的作用
Roles of Transcription Factor Gene OsPHR3 on the Utilization of Phosphate in Rice
摘要
Abstract
Phosphorus,an essential macronutrient element for plant growth and development,is broadly participating in various plant life activities.However,low availability of soil phosphate is a major constraint for crop production in man-y agricultural systems.OsPHR3(LOC_Os02g04640)is a homolog of OsPHR2,the central regulator of Pi-signaling in rice.Both OsPHR2 and OsPHR3 belong to the MYB-CC family members and have functional redundancy in part.In this study,OsPHR3 knock-out and over-expression transgenic plants were obtained.The hydroponic culture,32 Pi iso-topic and pot experiments were used to demonstrate the role of OsPHR3 in rice phosphate untilization.First,hydropo-nic experiments showed that over-expression of OsPHR3 increased the phosphorus content of the rice,although the knock-out lines had no significant difference with wild type plants.Second,32 Pi isotopic experiments revealed that over-expression of the gene promoted the absorption and distribution of phosphate,while the absorption rate in knock-out lines decreased in comparison with wild type plants under Pi-deficient condition.Third,pot experiments demonstrated that over-expression of OsPHR3 enhanced the effective tillers and phosphorus content in seed.However,the panicle length in knock-out lines is shorter than in wild type plants.In conclusion,OsPHR3 is a potential transcription factor for improving P efficiency in rice through molecular breeding.关键词
水稻/磷酸盐/OsP HR3Key words
rice/phosphate/OsPHR3分类
生物科学引用本文复制引用
张亮,孙文献,孙雅菲,裴文霞,罗闻真,孙瑞,张占田,徐国华,孙淑斌..水稻转录因子基因OsPHR3在磷素利用过程中的作用[J].中国水稻科学,2016,30(4):397-405,9.基金项目
国家自然科学基金资助项目(31172014) （31172014）
江苏省自然科学基金资助项目(BK20141367) （BK20141367）
转基因生物新品种培育科技重大专项(2014ZX0800931B （）
2016ZX08009-003-005) （）
