中国水稻科学2016，Vol.30Issue(4)：406-416,11.DOI:10.16819/j.1001-7216.2016.6026
类酵母共生菌中两个组氨酸合成基因在褐飞虱发育中的作用
Roles of Two Genes Involved in Histidine Biosynthetic Pathway in Yeast-Like Symbiont in Development of Nilaparvata lugens (Stål)
摘要
Abstract
Nilaparvata lugens is a serious phloem-feeding pest of rice in China.Based on genome and transcriptome data of N.lugens and yeast-like symbiont (YLS,also named Entomomyces delphacidicola str.NLU),the major biosynthesis pathways for amino acids in N .lugens were constructed.In this study,we cloned two genes,EdeHis2 and EdeHis6 ,which catalyze critical steps in histidine biosynthesis pathway,and revealed the negative effects of double-stranded RNA (dsRNA)on the growth,development and survival rate of N.lugens.Homology searches and phylogenetic analysis showed that EdeHis2 and EdeHis6 origin from YLS genome,share high similarities with that of Metarhizium acridum and formed a clad in the phylogenetic tree,whereas no His2- or His6-like genes was found in N .lugens genome.Temporal expression profiles of EdeHis2 and EdeHis6 showed that both genes were ubiquitously but unevenly expressed among the different life stages,and the spatial expression pattern showed they have higher expression levels in the fat body rather than head,leg,integument and midgut.Furthermore,no target products were amplified in head and wing genomic DNA of N.lugens,rather than that in abdomen genomic DNA.At two,four and six days after dsEdeHis2 or dsEdeHis6 injection,the mRNA abundance of target genes was decreased by 45%-60%(EdeHis2)or 27%-55% (EdeHis6),comparing with blank control.Down-regulation of EdeHis2 or dsEdeHis6 slightly increased the mortality by 8.3% or 9.2%,and delayed the nymphal duration of male and female by 0.43 and 0.65 day and 0.33 and 0.36 day,respectively.Moreover,both male (11%)and female adult (13%)showed wing deformation after inj ection of dsEdeHis6 ,higher than that in the blank control.In conclusion,EdeHis2 and EdeHis6 that origin from YLS were involved in histidine biosynthesis pathway,contributed to the survivor,development and wing formation of N.lugens.关键词
褐飞虱/组氨酸/类酵母共生菌/His2/His6/R N A干扰Key words
Nilaparvata lugens/yeast-like symbiont/histidine/His2/His6/RNAi分类
农业科技引用本文复制引用
唐耀华,万品俊,郝培应,傅强,俞晓平..类酵母共生菌中两个组氨酸合成基因在褐飞虱发育中的作用[J].中国水稻科学,2016,30(4):406-416,11.基金项目
国家自然科学基金资助项目(31371939) （31371939）
国家科技支撑计划资助项目(2012BAD19B03) （2012BAD19B03）
