中国水稻科学2016，Vol.30Issue(4)：441-446,6.DOI:10.16819/j.1001-7216.2016.5129
转磷酸甘露糖变位酶基因提高水稻维生素C含量
Transgenosis of the Phosphomannomutase Transgene Increases Vitamin C Content in Rice
摘要
Abstract
VC (vitamin C)is an essential nutrient to human health.Due to lack of L-gulonolactone oxidase,the last enzyme involved in VC synthesis pathway,human could not synthesize VC by themselves.Rice is an important food crop and its nutritional value could be greatly improved by increasing the VC content in rice seeds.Phosphomannomutas e (PMM)is an important enzyme in VC synthesis pathway,catalyzing the interconversion from mannose 6-phosphate from mannose 1-phosphate.In this study,Oryza PMM gene (OsPMM)that was under the control of seed-specific expressed promoter Bx14 was transferred into ‘C418’,a restorer line of three-line japonica hybrid rice,using the double right-border vector pMNDRBBin6 through an Agrobacterium tumefaciens-mediated system.Molecular analysis revealed that OsPMM was integrated into the genome of transgenic ‘C418’,and the homozygous and marker-free transgenic line was obtained in the T2 generation.Gene expression analysis of transgenic lines showed the expression level of OsPMM was significantly increased in seeds,and accordingly,the VC content in the seeds of transgenic plant also increased by 25-50%.关键词
维生素C/磷酸甘露糖变位酶/双右边界双元载体系统/无选择标记/转基因系Key words
vitamin C/phosphomannomutase/double right-border vector system/marker-free/transgenic line分类
生物科学引用本文复制引用
高利芬,夏志辉,张继,王道文,翟文学..转磷酸甘露糖变位酶基因提高水稻维生素C含量[J].中国水稻科学,2016,30(4):441-446,6.基金项目
国家转基因生物新品种培育重大专项(2014ZX08001-002) （2014ZX08001-002）
国家自然科学基金资助项目(31300999) （31300999）
湖北省教育厅项目(B2015229) （B2015229）
武汉市科学技术局项目(2014072704011250) （2014072704011250）
江汉大学科研启动项目(3003-06000043) （3003-06000043）
